College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
BGI-Hangzhou, Hangzhou, 310012, China.
Sci Data. 2023 Sep 14;10(1):627. doi: 10.1038/s41597-023-02533-0.
Axolotl (Ambystoma mexicanum) is an excellent model for investigating regeneration, the interaction between regenerative and developmental processes, comparative genomics, and evolution. The brain, which serves as the material basis of consciousness, learning, memory, and behavior, is the most complex and advanced organ in axolotl. The modulation of transcription factors is a crucial aspect in determining the function of diverse regions within the brain. There is, however, no comprehensive understanding of the gene regulatory network of axolotl brain regions. Here, we utilized single-cell ATAC sequencing to generate the chromatin accessibility landscapes of 81,199 cells from the olfactory bulb, telencephalon, diencephalon and mesencephalon, hypothalamus and pituitary, and the rhombencephalon. Based on these data, we identified key transcription factors specific to distinct cell types and compared cell type functions across brain regions. Our results provide a foundation for comprehensive analysis of gene regulatory programs, which are valuable for future studies of axolotl brain development, regeneration, and evolution, as well as on the mechanisms underlying cell-type diversity in vertebrate brains.
美西螈（Ambystoma mexicanum）是研究再生、再生和发育过程相互作用、比较基因组学和进化的优秀模型。大脑作为意识、学习、记忆和行为的物质基础，是美西螈中最复杂和先进的器官。转录因子的调节是决定大脑不同区域功能的关键方面。然而，我们对美西螈脑区的基因调控网络还没有全面的了解。在这里，我们利用单细胞 ATAC 测序技术，生成了嗅球、端脑、间脑和中脑、下丘脑和垂体以及后脑 81199 个细胞的染色质可及性图谱。基于这些数据，我们鉴定了特定于不同细胞类型的关键转录因子，并比较了不同脑区的细胞类型功能。我们的研究结果为全面分析基因调控程序提供了基础，这对于未来研究美西螈大脑发育、再生和进化以及脊椎动物大脑中细胞类型多样性的机制具有重要价值。
