Johnson L, Petty C S, Neaves W B
J Androl. 1986 Sep-Oct;7(5):316-22. doi: 10.1002/j.1939-4640.1986.tb00939.x.
Tubular boundary tissue and seminiferous epithelia were evaluated stereologically in testes from 28 men aged 20 to 48 years and 28 men aged 50 to 90 years. Testes obtained at autopsy within 15 hours of death were perfused with glutaraldehyde, embedded in Epon (Ladd Research Industries, Inc., Burlington, VT), sectioned at 0.5 micron, and stained with toluidine blue. Volume densities (percentage of the testicular parenchyma) of various parameters determined by point counting and diameter measurements were used to calculate total volumes, length of tubules, and number of cells. Electron microscopy was used to determine the volume density of myoid cells in the boundary tissue. Significant (P less than 0.01) age-related reductions occurred in paired testicular weights, paired parenchymal weights, total volume of seminiferous tubules and of seminiferous epithelium, and length of tubules. The volume density and thickness of boundary tissue increased (P less than 0.01) with age. The volume of boundary tissue per man and the volume density of myoid cells in the boundary tissue did not vary with age. Although the number of myoid cells per man tended to be lower in the older group, the number of myoid cells per cross section of seminiferous tubule was increased (P less than 0.01) in older men. The age-related thickening of the boundary tissue was not due to an increase in boundary tissue but resulted from a reduction in the length of the seminiferous tubules.
对28名年龄在20至48岁之间的男性以及28名年龄在50至90岁之间的男性的睾丸进行了组织学评估，观察其管状边界组织和生精上皮。在死亡后15小时内尸检获得的睾丸用戊二醛灌注，包埋在Epon（Ladd Research Industries, Inc., Burlington, VT）中，切成0.5微米厚的切片，并用甲苯胺蓝染色。通过点计数和直径测量确定的各种参数的体积密度（睾丸实质的百分比）用于计算总体积、小管长度和细胞数量。电子显微镜用于确定边界组织中肌样细胞的体积密度。成对睾丸重量、成对实质重量、生精小管和生精上皮的总体积以及小管长度均出现了与年龄相关的显著降低（P<0.01）。边界组织的体积密度和厚度随年龄增加（P<0.01）。每人的边界组织体积和边界组织中肌样细胞的体积密度不随年龄变化。尽管老年组每人的肌样细胞数量往往较低，但老年男性生精小管每个横截面上的肌样细胞数量增加（P<0.01）。与年龄相关的边界组织增厚并非由于边界组织增加，而是由于生精小管长度减少所致。
