Sacchettini J C, Meininger T, Roderick S, Banaszak L J
J Biol Chem. 1986 Nov 15;261(32):15183-5.
Single crystals of fumarase purified from pig heart have been prepared from solutions containing polyethylene glycol. The crystals give diffraction data corresponding to Bragg spacings of 2.0 A and contain a single subunit of the enzyme in the asymmetric unit of the C222 unit cell. Therefore, the subunits of this tetrameric molecule are arranged with the point symmetry group 222. The present purification scheme and studies of the NH2-terminal amino acid sequences suggest that only a single form of the enzyme is present, and it is thought to be the mitochondrial enzyme.
从含有聚乙二醇的溶液中制备了从猪心纯化的延胡索酸酶单晶。这些晶体给出了对应于2.0埃布拉格间距的衍射数据，并且在C222晶胞的不对称单元中包含该酶的一个亚基。因此，这个四聚体分子的亚基以点对称群222排列。目前的纯化方案和对NH2末端氨基酸序列的研究表明只存在一种形式的该酶，并且认为它是线粒体酶。
