Weaver T M, Levitt D G, Banaszak L J
Department of Biochemistry, University of Minnesota, Minneapolis 55455.
J Mol Biol. 1993 May 5;231(1):141-4. doi: 10.1006/jmbi.1993.1264.
Fumarase C purified from Escherichia coli has been crystallized in the presence of polyethylene glycol in both a citrate buffer at pH 5.3 and a 3-(4-morpholino)-propanesulfonic acid buffer at pH 7.5 yielding two crystal forms. An orthorhombic C222(1) form was obtained in citrate at pH 5.3 and an orthorhombic I222 form was obtained in 3-(4-morpholino)-propanesulfonic acid (pH 7.5). Complete native data sets have been collected on both crystal forms: the C222(1) form is complete to 2.10 A and the I222 form is complete to 2.20 A.
从大肠杆菌中纯化的延胡索酸酶C在聚乙二醇存在下，于pH 5.3的柠檬酸盐缓冲液和pH 7.5的3-（4-吗啉基）-丙磺酸缓冲液中结晶，产生了两种晶体形式。在pH 5.3的柠檬酸盐中获得了正交晶系C222(1)形式，在3-（4-吗啉基）-丙磺酸（pH 7.5）中获得了正交晶系I222形式。已收集了两种晶体形式的完整原生数据集：C222(1)形式完整至2.10 Å，I222形式完整至2.20 Å。
