University of Pittsburgh, Department of Bioengineering, 4200 Fifth Avenue, Pittsburgh, PA, 15260, USA; Center for the Neural Basis of Cognition, 4400 Fifth Avenue, Suite 115, Pittsburgh, PA, 15213, USA.
University of Pittsburgh, Department of Bioengineering, 4200 Fifth Avenue, Pittsburgh, PA, 15260, USA; Center for the Neural Basis of Cognition, 4400 Fifth Avenue, Suite 115, Pittsburgh, PA, 15213, USA; McGowan Institute for Regenerative Medicine, 450 Technology Drive, Suite 300, Pittsburgh, PA, 15219, USA.
Biomaterials. 2023 Nov;302:122326. doi: 10.1016/j.biomaterials.2023.122326. Epub 2023 Sep 12.
We developed a nanoparticle base layer technology capable of maintaining the bioactivity of protein-based neural probe coating intended to improve neural recording quality. When covalently bound on thiolated nanoparticle (TNP) modified surfaces, neural adhesion molecule L1 maintained bioactivity throughout 8 weeks of dry storage at room temperature, while those bound to unmodified surfaces lost 66% bioactivity within 3 days. We tested the TNP + L1 coating in mouse brains on two different neural electrode arrays after two different dry storage durations (3 and 28 days). The results show that dry-stored coating is as good as the freshly prepared, and even after 28 days of storage, the number of single units per channel and signal-to-noise ratio of the TNP + L1 coated arrays were significantly higher by 32% and 40% respectively than uncoated controls over 16 weeks. This nanoparticle base layer approach enables the dissemination of biomolecule-functionalized neural probes to users worldwide and may also benefit a broad range of applications that rely on surface-bound biomolecules.
我们开发了一种纳米颗粒底层技术，能够保持基于蛋白质的神经探针涂层的生物活性，旨在提高神经记录质量。当共价结合到巯基化纳米颗粒 (TNP) 修饰的表面时，神经黏附分子 L1 在室温下干燥储存 8 周内保持生物活性，而结合到未修饰表面的则在 3 天内失去 66%的生物活性。我们在两种不同的神经电极阵列上测试了 TNP+L1 涂层，这些阵列在两种不同的干燥储存时间（3 天和 28 天）后进行了测试。结果表明，干燥储存的涂层与新制备的一样好，即使在储存 28 天后，TNP+L1 涂层阵列的每个通道的单个单位数量和信号噪声比分别比未涂层对照高出 32%和 40%，超过 16 周。这种纳米颗粒底层方法使具有生物分子功能化的神经探针能够在全球范围内传播给用户，也可能有益于依赖表面结合生物分子的广泛应用。
