Nicot G, Lachatre G, Gonnet C, Mallon J, Mocaer E
J Chromatogr. 1986 Aug 22;381(1):115-26. doi: 10.1016/s0378-4347(00)83570-7.
An isocratic reversed-phase ion-pair liquid chromatographic method for the determination of tianeptine and its two main metabolites in plasma, urine and tissues, using an internal standard, is reported. The influence of two stationary phases on the retention of the drugs was studied. The drugs were extracted as ion pairs, using a heptane-octanol-tetraheptylammonium bromide mixture (98:2:0.5, v/v/w) as extraction solvent. This extraction procedure yielded plasma drug recoveries of greater than 60% and allowed UV detection at 220 nm without interference from endogenous components of plasma, urine or tissues. Linear standard curves up to 1.00 micrograms/ml and drug determination down to 0.01 microgram/ml were observed. This method has been successfully applied to the analysis of human plasma and urine samples and of encephales from tianeptine-dosed rats.
报道了一种等度反相离子对液相色谱法，用于测定血浆、尿液和组织中的噻奈普汀及其两种主要代谢物，采用内标法。研究了两种固定相对药物保留的影响。药物以离子对形式萃取，使用庚烷-辛醇-四庚基溴化铵混合物（98:2:0.5，v/v/w）作为萃取溶剂。该萃取方法使血浆药物回收率大于60%，并允许在220 nm处进行紫外检测，而不受血浆、尿液或组织内源性成分的干扰。观察到线性标准曲线高达1.00微克/毫升，药物测定下限为0.01微克/毫升。该方法已成功应用于分析人类血浆和尿液样本以及来自给予噻奈普汀的大鼠的脑样本。
