高效液相色谱法测定人血浆中阿米替林及其主要代谢产物

High-performance liquid chromatographic method for the determination of amineptine and its main metabolite in human plasma.

作者信息

Nicot G, Lachatre G, Gonnet C, Valette J P, Merle L, Nouaille Y, Bromet N

出版信息

J Chromatogr. 1984 Mar 9;306:279-90. doi: 10.1016/s0378-4347(00)80890-7.

Abstract

An isocratic reversed-phase ion-pair liquid chromatographic method for the determination of amineptine and its main metabolite in plasma using an internal standard, is reported. The effects of stationary phase alkyl chain length and the concentration of alkyl sulfonate in the mobile phase were investigated. The drugs were extracted as ion pairs and the influence of various parameters on the extraction efficiency are discussed. Using a heptane-octanol-tetraheptylammonium bromide mixture (98:2:0.5, v/v/w) as extraction solvent, more than 60% of each drug was recovered with a very good selectivity. UV detection at 220 nm allowed drug determination down to 0.010 microgram/ml. Linear standard curves up to 1.00 microgram/ml were observed.

摘要

报道了一种等度反相离子对液相色谱法，用于测定血浆中阿米替林及其主要代谢物，采用内标法。研究了固定相烷基链长度和流动相中烷基磺酸盐浓度的影响。药物以离子对形式萃取，并讨论了各种参数对萃取效率的影响。使用庚烷-辛醇-四庚基溴化铵混合物（98:2:0.5，v/v/w）作为萃取溶剂，每种药物的回收率超过60%，选择性良好。在220 nm处进行紫外检测，可测定低至0.010微克/毫升的药物。观察到线性标准曲线高达1.00微克/毫升。