采用高效液相色谱-荧光检测法测定大鼠松果体中褪黑素和血清素含量随性别及发情周期的变化。

Variation of melatonin and serotonin content in rat pineal gland with sex and oestrous phase difference determined by high-performance liquid chromatography with fluorimetric detection.

作者信息

Wakabayashi H, Shimada K, Aizawa Y

出版信息

J Chromatogr. 1986 Aug 22;381(1):21-8. doi: 10.1016/s0378-4347(00)83561-6.

Abstract

Simultaneous determination of melatonin and serotonin in rat pineal gland is described using reversed-phase high-performance liquid chromatography with fluorimetric detection. These indoles were analysed isocratically within 15 min. In this work, veratric acid (3,4-dimethoxybenzoic acid), which has fluorescence characteristics (lambda ex = 290 nm, lambda em = 350 nm) around the wavelength of native fluorescence of melatonin (lambda ex = 285 nm, lambda em = 345 nm), was used as an internal standard. This method was applied to the determination of melatonin and serotonin in male and female rat pineal gland. No significant differences between the two groups were observed in the pineal melatonin and serotonin contents. The pineal melatonin and serotonin contents were compared with the oestrous and the di-oestrous phases of female rats. They were not widely different from each other.

摘要

采用反相高效液相色谱荧光检测法同时测定大鼠松果体中的褪黑素和5-羟色胺。这些吲哚类物质在15分钟内等度分析。在这项工作中，具有荧光特性（激发波长λex = 290 nm，发射波长λem = 350 nm）且在褪黑素天然荧光波长（激发波长λex = 285 nm，发射波长λem = 345 nm）附近的藜芦酸（3,4-二甲氧基苯甲酸）被用作内标。该方法用于测定雄性和雌性大鼠松果体中的褪黑素和5-羟色胺。两组之间的松果体褪黑素和5-羟色胺含量未观察到显著差异。将松果体褪黑素和5-羟色胺含量与雌性大鼠的发情期和间情期进行比较。它们彼此之间差异不大。