Harumi T, Akutsu H, Matsushima S
Department of Anatomy, Asahikawa Medical College, Japan.
J Chromatogr B Biomed Appl. 1996 Jan 12;675(1):152-6. doi: 10.1016/0378-4347(95)00345-2.
A simple and simultaneous determination of melatonin and its precursors, serotonin (5-HT) and N-acetylserotonin, was achieved by reversed-phase high-performance liquid chromatography with electrochemical detection. The addition of an ion-pairing agent, sodium 1-octanesulfonate, to the chromatographic mobile phase caused an increase of the retention time of 5-HT, and resulted in the successful simultaneous resolution of these three indoleamines. This method was used to quantitate these indoleamines in the pineal gland of juvenile golden hamsters.
通过反相高效液相色谱-电化学检测法实现了褪黑素及其前体血清素（5-羟色胺，5-HT）和N-乙酰血清素的简单同时测定。向色谱流动相中添加离子对试剂1-辛烷磺酸钠会使5-HT的保留时间增加，并成功实现了这三种吲哚胺的同时分离。该方法用于定量幼年金黄仓鼠松果体中的这些吲哚胺。
