Mefford I N, Barchas J D
J Chromatogr. 1980 Feb 8;181(2):187-93. doi: 10.1016/s0378-4347(00)81604-7.
Tryptophan and many of its indole metabolites were separated using reversed-phase high-performance liquid chromatography (HPLC) and determined using electrochemical detection. This was accomplished isocratically using an acetate--citric acid eluent with various amounts of methanol. Brain and pineal tissue was analyzed for several tryptophan metabolites. Tissue preparation required only homogenization in acidic solution and centrifugation prior to application to the HPLC column. Detection limits in the low picogram range were found for those indoles separated.
色氨酸及其许多吲哚代谢产物通过反相高效液相色谱（HPLC）进行分离，并使用电化学检测法进行测定。这是通过使用含有不同量甲醇的醋酸盐 - 柠檬酸洗脱液等度洗脱来完成的。对脑和松果体组织中的几种色氨酸代谢产物进行了分析。组织制备仅需在酸性溶液中匀浆并在应用于HPLC柱之前进行离心。对于所分离的那些吲哚，检测限在低皮克范围内。
