Zhao Hui, Wang Yang, Xu Hui, Liu Meng, Xu Xinmei, Zhu Sijing, Liu Zhao, Cai Han, Wang Yinan, Lu Jinhua, Yang Xiaoqing, Kong Shuangbo, Bao Haili, Wang Haibin, Deng Wenbo
Fujian Provincial Key Laboratory of Reproductive Health Research, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, Fujian 361102, China.
State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, School of Medicine, Xiamen University, Xiamen, Fujian, China.
iScience. 2023 Sep 1;26(10):107796. doi: 10.1016/j.isci.2023.107796. eCollection 2023 Oct 20.
The underlying mechanisms governing parturition remain largely elusive due to limited knowledge of parturition preparation and initiation. Accumulated evidences indicate that maternal decidua plays a critical role in parturition initiation. To comprehensively decrypt the cell heterogeneity in decidua approaching parturition, we investigate the roles of various cell types in mouse decidua process and reveal previously unappreciated insights in parturition initiation utilizing single-cell RNA sequencing (scRNA-seq). We enumerate the cell types in decidua and identity five different stromal cells populations and one decidualized stromal cells. Furthermore, our study unravels that stromal cells prepare for parturition by regulating local retinol acid (RA) synthesis. RA supplement decreases expression of extracellular matrix-related genes and accelerates the timing of parturition . Collectively, the discovery of contribution of stromal cells in parturition expands current knowledge about parturition and opens up avenues for the intervention of preterm birth (PTB).
由于对分娩准备和启动的了解有限，控制分娩的潜在机制在很大程度上仍然难以捉摸。越来越多的证据表明，母体蜕膜在分娩启动中起着关键作用。为了全面解密接近分娩时蜕膜中的细胞异质性，我们研究了各种细胞类型在小鼠蜕膜过程中的作用，并利用单细胞RNA测序（scRNA-seq）揭示了分娩启动中以前未被重视的见解。我们列举了蜕膜中的细胞类型，鉴定出五种不同的基质细胞群体和一种蜕膜化基质细胞。此外，我们的研究揭示，基质细胞通过调节局部视黄酸（RA）合成来为分娩做准备。补充RA可降低细胞外基质相关基因的表达，并加速分娩时间。总的来说，基质细胞在分娩中的作用的发现扩展了当前关于分娩的知识，并为早产（PTB）的干预开辟了途径。
