乙酰化木犀草素和棉酚抑制免疫复合物介导的中性粒细胞活化和大疱性表皮松解症获得性模型中的表皮-真皮分离。
Luteolin peracetate and gossypolone inhibit immune complex-mediated neutrophil activation and dermal-epidermal separation in an model of epidermolysis bullosa acquisita.
机构信息
Priority Area Chronic Lung Diseases, Research Center Borstel, Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Borstel, Germany.
Lübeck Institute of Experimental Dermatology and Center for Research on Inflammation of the Skin, University of Lübeck, Lübeck, Germany.
出版信息
Front Immunol. 2023 Sep 1;14:1196116. doi: 10.3389/fimmu.2023.1196116. eCollection 2023.
INTRODUCTION
Natural products have been shown to an important source of therapeutics for human disease. In this study, we aimed to identify natural compounds as potential therapeutics for epidermolysis bullosa acquisita (EBA), an autoimmune disease caused by autoantibodies to type VII collagen (COL7).
METHODS
Utilizing an experimental system, we screened a natural product library composed of 800 pure compounds for their inhibitory effect on COL7-anti-COL7 IgG immune complex (IC)-mediated neutrophil activation and on neutrophil-mediated tissue damage.
RESULTS
Three natural compounds, namely luteolin peracetate, gossypol, and gossypolone were capable in inhibiting the IC-induced neutrophil adhesion and oxygen burst . Furthermore, luteolin peracetate and gossypolone were able to inhibit the anti-COL7 IgG induced dermal-epidermal separation in an model for EBA.
DISCUSSION
In summary, this study demonstrates that luteolin peracetate and gossypolone are potential therapeutics for experimental EBA, which deserves further investigation.
简介
天然产物已被证明是治疗人类疾病的重要来源。在这项研究中，我们旨在鉴定天然化合物作为获得性大疱性表皮松解症（EBA）的潜在治疗药物，EBA 是一种由 VII 型胶原（COL7）自身抗体引起的自身免疫性疾病。
方法
利用实验系统，我们筛选了由 800 种纯化合物组成的天然产物文库，以研究它们对 COL7-抗 COL7 IgG 免疫复合物（IC）介导的中性粒细胞激活和中性粒细胞介导的组织损伤的抑制作用。
结果
三种天然化合物，即乙酰化木樨草素、棉酚和棉酚酮，能够抑制 IC 诱导的中性粒细胞黏附和氧爆发。此外，乙酰化木樨草素和棉酚酮能够抑制 EBA 模型中抗 COL7 IgG 诱导的真皮-表皮分离。
讨论
总之，本研究表明乙酰化木樨草素和棉酚酮是实验性 EBA 的潜在治疗药物，值得进一步研究。
Zotero 插件