Rojas-Orduña Elkin, Hernández-Carrión María, Gómez-Franco Juan David, Narváez-Cuenca Carlos-Eduardo, Sánchez-Camargo Andrea Del Pilar
Group of Product and Process Design, Department of Chemical and Food Engineering, Universidad de los Andes, Bogotá, Colombia.
Food Chemistry Research Group, Departamento de Química, Facultad de Ciencias, Universidad Nacional de Colombia, Bogotá, Colombia.
Front Nutr. 2023 Aug 31;10:1231049. doi: 10.3389/fnut.2023.1231049. eCollection 2023.
This study aimed to investigate the macronutrient and carotenoid content of red and yellow var. Caturra pulp, a by-product of coffee processing in Colombia. The study employed ultra-sound-assisted extraction (UAE) to extract carotenoids, and a 2 factorial design was used to evaluate the effects of pulp color, biomass-solvent ratio, and solvent mixture composition on carotenoid content and extraction yield. The condition that provided the highest carotenoid extraction was further encapsulated by spray drying and added to a dairy product. The results showed that coffee pulp has significant dietary fiber content and high levels of carotenoids, with yellow pulp having a higher content than red pulp. Lutein isomers and lutein esters were the most abundant carotenoids found in both red and yellow coffee pulp. The highest carotenoid extraction was achieved using a 1:40 (g/mL) biomass:solvent ratio and a 20:80% v/v Ethanol:Ethyl Acetate solvent mixture for the yellow pulp. The carotenoid extract also demonstrated high encapsulation efficiency (46.57 ± 4.03%) and was found to be stable when added to a fermented milk product. This study presents an alternative solution for utilizing coffee by-products in Colombia, which could positively impact the families of over half a million Colombian coffee producers.
本研究旨在调查哥伦比亚咖啡加工副产品——红色和黄色卡杜拉品种果肉中的常量营养素和类胡萝卜素含量。该研究采用超声辅助萃取(UAE)来提取类胡萝卜素,并使用二因素设计来评估果肉颜色、生物质-溶剂比和溶剂混合物组成对类胡萝卜素含量和萃取产率的影响。通过喷雾干燥对提供最高类胡萝卜素萃取量的条件下获得的提取物进行进一步包封,并添加到乳制品中。结果表明,咖啡果肉含有大量膳食纤维和高水平的类胡萝卜素,黄色果肉中的含量高于红色果肉。叶黄素异构体和叶黄素酯是在红色和黄色咖啡果肉中发现的最丰富的类胡萝卜素。对于黄色果肉,使用1:40(g/mL)的生物质:溶剂比和20:80% v/v的乙醇:乙酸乙酯溶剂混合物可实现最高的类胡萝卜素萃取量。类胡萝卜素提取物还表现出高包封效率(46.57±4.03%),并且在添加到发酵乳制品中时被发现是稳定的。本研究为哥伦比亚咖啡副产品的利用提供了一种替代解决方案,这可能对超过五十万哥伦比亚咖啡生产者的家庭产生积极影响。