Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE), Edificio Torregaitán, Universidad Miguel Hernández de Elche (UMH), Avenida de la Universidad s/n, 03202 Elche, Alicante, Spain.
Departamento de Agroquímica y Medio Ambiente, Universidad Miguel Hernández de Elche (UMH), Campus de Orihuela, Carretera de Beniel km 3.2, 03312 Orihuela, Alicante, Spain.
Food Res Int. 2021 Mar;141:109882. doi: 10.1016/j.foodres.2020.109882. Epub 2020 Nov 10.
The aim of this study was the use and revalorization of two persimmon by-products A and B generated in the juice production process. The by-product B resulting from a pectinase enzymatic treatment of peels and pulp to optimize juice extraction was especially suitable for recovery of valuable bioactive carotenoids. The extraction solvents and solvent combinations used were: ethanol, acetone, ethanol/acetone (50:50 v/v) and ethanol/acetone/hexane (25:25:50 v/v/v). HPLC-DAD analysis detected and identified a total of nine individual carotenoids namely violaxanthin, neoxanthin, antheraxanthin, lutein, zeaxanthin, β-cryptoxanthin 5,6-epoxide, β-cryptoxanthin, α-carotene, and β-carotene. β-cryptoxanthin and β-carotene represented 49.2% and 13.2% of the total carotenoid content (TCC) in the acetone extract from by-product B. TCC contributed greatly to antioxidant activity of acetone extract derived from this by-product. Pectinase enzymatic treatment of persimmon peels and pulp followed by absolute acetone extraction of carotenoids could be an efficient method to obtain a rich extract in these compounds that could be used as nutraceutical ingredient.
本研究的目的是利用和再利用在果汁生产过程中产生的两种柿子副产品 A 和 B。副产品 B 是通过果胶酶处理果皮和果肉以优化果汁提取而产生的，特别适合回收有价值的生物活性类胡萝卜素。所用的提取溶剂和溶剂组合为：乙醇、丙酮、乙醇/丙酮（50:50v/v）和乙醇/丙酮/己烷（25:25:50v/v/v）。HPLC-DAD 分析共检测和鉴定了九种单独的类胡萝卜素，即 violaxanthin、neoxanthin、antheraxanthin、叶黄素、玉米黄质、β-隐黄质 5,6-环氧化物、β-隐黄质、α-胡萝卜素和β-胡萝卜素。β-隐黄质和β-胡萝卜素分别占副产品 B 中丙酮提取物总类胡萝卜素含量（TCC）的 49.2%和 13.2%。TCC 对来源于该副产品的丙酮提取物的抗氧化活性有很大贡献。果胶酶处理柿子果皮和果肉，然后用绝对丙酮提取类胡萝卜素，可能是获得富含这些化合物的提取物的有效方法，可作为营养保健品成分使用。
