负重训练可缓解中年大鼠的肌肉萎缩和细胞焦亡。
Weight bearing training alleviates muscle atrophy and pyroptosis of middle-aged rats.
机构信息
Department of Physical Education, Northwestern Polytechnical University, Xi'an, China.
Key Laboratory of Physical Fitness and Exercise of Ministry of Education, Beijing Sport University, Beijing, China.
出版信息
Front Endocrinol (Lausanne). 2023 Aug 30;14:1202686. doi: 10.3389/fendo.2023.1202686. eCollection 2023.
BACKGROUND
Age-related muscle atrophy and adipose accumulation begin to occur in young and middle-aged individuals, and exercise at an early age improves body composition. Pyroptosis may play an essential role in age-related low-grade inflammation. This study aimed to explore the alleviation of muscle atrophy by weight-bearing training with increasing age inhibition of pyroptosis.
METHODS
Ninety 8-month-old male SD rats were randomly divided into three groups: (1) normal baseline group (N group, = 10), sacrificed after adaptive feeding; control group (C group, = 40); and weight-bearing running group (R group, = 40). Blood samples, adipose tissue (AT), and extensor digitorum longus (EDL) were collected after 8, 16, 24, and 32-weeks intervention.
RESULTS
The body weight, muscle mass, fat mass, plasma lipid, AT wet weight, adipocyte cross-sectional area (CSA), and apoptosis rates of AT and EDL were increased, while the muscle mass, wet weight, and fiber CSA of EDL were decreased by aging, which were reversed by exercise. Weight-bearing training promoted protein synthesis in EDL, inhibited protein degradation in EDL, and expression of pyroptotic key proteins in EDL and AT in rats.
CONCLUSION
Weight-bearing training improves body composition and alleviates age-related muscle atrophy in rats, and its mechanism may be related to the inhibition of pyroptosis in the EDL and AT and the improvement of muscle protein metabolism.
背景
与年龄相关的肌肉萎缩和脂肪堆积开始发生在中青年人群中，年轻时进行运动可以改善身体成分。细胞焦亡可能在与年龄相关的低度炎症中发挥重要作用。本研究旨在探索负重训练对年龄相关肌肉萎缩的缓解作用，抑制细胞焦亡。
方法
90 只 8 月龄雄性 SD 大鼠随机分为三组：（1）正常基线组（N 组，n=10），适应性喂养后处死；对照组（C 组，n=40）；负重跑组（R 组，n=40）。干预 8、16、24 和 32 周后，采集血样、脂肪组织（AT）和趾长伸肌（EDL）。
结果
随着年龄的增长，体重、肌肉质量、脂肪质量、血浆脂质、AT 湿重、脂肪细胞横截面积（CSA）和 AT 和 EDL 的细胞凋亡率增加，而 EDL 的肌肉质量、湿重和纤维 CSA 减少，但运动可以逆转这些变化。负重训练促进了 EDL 中的蛋白质合成，抑制了 EDL 中的蛋白质降解，以及 EDL 和 AT 中细胞焦亡关键蛋白的表达。
结论
负重训练改善了大鼠的身体成分，缓解了与年龄相关的肌肉萎缩，其机制可能与 EDL 和 AT 中的细胞焦亡抑制以及肌肉蛋白代谢的改善有关。
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