Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, GDMPA Key Laboratory for Process Control and Quality Evaluation of Chiral Pharmaceuticals, School of Chemistry, South China Normal University, Guangzhou, P. R. China.
Anal Methods. 2023 Sep 28;15(37):4946-4953. doi: 10.1039/d3ay01260c.
As an estrogenic mycotoxin found in a wide range of agricultural crops, the toxicity of zearalenone (ZEN) poses a serious risk to human health. Accordingly, to achieve rapid detection of zearalenone in complex samples, an aptamer fluorescence sensor based on magnetic graphene oxide was developed. Compared with traditional methods, this technique has the virtues of simple operation, low cost, and reliable performance. Magnetic graphene oxide (MGO) was synthesized as a fluorescent bursting agent, using a chemical precipitation approach by depositing FeO on the surface of graphene oxide. As a fluorescent probe, an aptamer coupling with CdTe quantum dots and zearalenone was used. Following the specific binding of zearalenone and the aptamer, the affinity interaction between the fluorescent probe and MGO was weakened, resulting in the recovery of fluorescence and making the qualitative and quantitative analysis of zearalenone available fluorescence intensity determination. The results indicated that the method's linear range was 5-120 μg L and its detection limit was 2.9 μg L. In addition, the recoveries varied from 76.4 to 118.8% for crop samples, with relative standard deviations (RSDs) between 0.8 and 9.5%, which suggests an effective method for the separation and detection of mycotoxins in actual environmental samples.
作为一种广泛存在于各种农作物中的雌激素真菌毒素，玉米赤霉烯酮（ZEN）的毒性对人类健康构成了严重威胁。因此，为了实现对复杂样品中玉米赤霉烯酮的快速检测，开发了一种基于磁性氧化石墨烯的适体荧光传感器。与传统方法相比，该技术具有操作简单、成本低、性能可靠等优点。磁性氧化石墨烯（MGO）是通过在氧化石墨烯表面沉淀 FeO 合成的荧光猝灭剂。作为荧光探针，使用与 CdTe 量子点和玉米赤霉烯酮偶联的适体。玉米赤霉烯酮与适体特异性结合后，荧光探针与 MGO 之间的亲和相互作用减弱，导致荧光恢复，从而实现玉米赤霉烯酮的定性和定量分析，通过荧光强度测定。结果表明，该方法的线性范围为 5-120 μg L，检测限为 2.9 μg L。此外，作物样品的回收率在 76.4%至 118.8%之间，相对标准偏差（RSD）在 0.8%至 9.5%之间，这表明该方法可有效分离和检测实际环境样品中的真菌毒素。
