Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, High-tech Key Laboratory of Agricultural Equipment & Intelligentization of Jiangsu Province, School of Agricultural Equipment Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China; Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, PA 19104, United States.
Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, High-tech Key Laboratory of Agricultural Equipment & Intelligentization of Jiangsu Province, School of Agricultural Equipment Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Food Chem. 2020 Aug 30;322:126778. doi: 10.1016/j.foodchem.2020.126778. Epub 2020 Apr 8.
Zearalenone (ZEN) is highly toxic to humans, and therefore, development of sensitive and effective methods for ZEN quantification in cereal crops is particularly important. Here, an innovative photoelectrochemical (PEC) aptasensor based on simply in-situ conjugated composites of zinc oxide-nitrogen doped graphene quantum dots (ZnO-NGQDs) was constructed. On addition of NGQDs, the composites displayed higher PEC signal with 8.8-fold enhancement than pure ZnO nanoparticles. A sensitive and selective PEC aptasensor was fabricated by combining the composites with ZEN aptamer, which yielded an excellent analytical performance for ZEN detection, with a wide linear range of 1.0 × 10-1.0 × 10 g mL and a low detection limit of 3.3 × 10 g mL. Good recoveries were obtained using the PEC aptasensor, which were consistent with those obtained using the national standard method (HPLC-MS). Finally, ZEN in mildewing cereal crops was monitored with the PEC aptasensor, exhibiting good potential for application in cereal crops for early diagnosis.
玉米赤霉烯酮（ZEN）对人类具有高毒性，因此，开发用于谷物中 ZEN 定量的敏感且有效的方法尤为重要。在此，构建了一种基于氧化锌-氮掺杂石墨烯量子点（ZnO-NGQDs）的简单原位共轭复合材料的创新光电化学（PEC）适体传感器。加入 NGQDs 后，复合材料的 PEC 信号增强了 8.8 倍，比纯 ZnO 纳米粒子高。通过将复合材料与 ZEN 适体结合，构建了一种灵敏且选择性的 PEC 适体传感器，用于 ZEN 检测，具有 1.0×10-1.0×10g mL 的宽线性范围和 3.3×10g mL 的低检测限。使用 PEC 适体传感器获得了良好的回收率，与国家标准方法（HPLC-MS）一致。最后，使用 PEC 适体传感器监测了霉变谷物中的 ZEN，展示了在谷物早期诊断中应用的良好潜力。
