Alfredo Maldonado-Barrueco, Clinical Microbiology Department. Hospital Universitario La Paz. Madrid, Spain.
Rev Esp Quimioter. 2023 Dec;36(6):625-628. doi: 10.37201/req/058.2023. Epub 2023 Sep 18.
The prevalence of drug-resistant Neisseria gonorrhoeae (NG) infections is increasing. Studies report the prevalence of NG strains presenting A2059G/C2611T (rRNA23S) and S91F (parC) mutations conferring resistance to azithromycin and ciprofloxacin.
We conducted a prospective cohort study evaluating first void-urine urines, rectal, and oropharyngeal swabs collected from a cohort of patients in a tertiary hospital in Madrid between October 2022 and January 2023. Samples were screened by Allplex 7-STI Essential Assay (Seegene). Drug resistances were performed by Allplex NG-DR Assay (Seegene).
A total of 1,415 patients were included, of which 112 had a positive sample for NG infection. One patient had a C2611T mutation (0.9%) and neither patient showed A2059G mutation. We found 67 (59.8%) S91F-positive patients. Forty-four patients (39.3%) not had any mutations.
We report a low-prevalence of mutations A2059G/C2611T to macrolides and a high-prevalence to S91F in NG infections. Molecular methods for the detection of NG resistance could be useful in direct non-culturable samples.
耐多药淋病奈瑟菌（NG）感染的流行率正在上升。研究报告称，存在 A2059G/C2611T（rRNA23S）和 S91F（parC）突变的 NG 菌株的流行率增加，这些突变导致对阿奇霉素和环丙沙星的耐药性。
我们进行了一项前瞻性队列研究，评估了 2022 年 10 月至 2023 年 1 月期间马德里一家三级医院的患者队列的首次晨尿、直肠和咽拭子。使用 Allplex 7-STI Essential 检测试剂盒（Seegene）对样本进行筛查。使用 Allplex NG-DR 检测试剂盒（Seegene）进行药物耐药性检测。
共纳入 1415 例患者，其中 112 例 NG 感染阳性。1 例患者存在 C2611T 突变（0.9%），无患者存在 A2059G 突变。我们发现 67 例（59.8%）S91F 阳性患者。44 例（39.3%）患者没有任何突变。
我们报告了 NG 感染中大环内酯类药物耐药性 A2059G/C2611T 突变的低流行率和 S91F 突变的高流行率。用于检测 NG 耐药性的分子方法可能对非培养直接样本有用。
