黄曲霉菌素和电离γ射线对人喉癌细胞系的双重抗癌活性。

Dual anticancer activity of Aspergillus nidulans pigment and Ionizing γ-Radiation on human larynx carcinoma cell line.

机构信息

The Regional Center for Mycology and Biotechnology (RCMB), Al-Azhar University, Cairo, Egypt.

Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt.

出版信息

BMC Complement Med Ther. 2023 Sep 18;23(1):327. doi: 10.1186/s12906-023-04162-x.

DOI:10.1186/s12906-023-04162-x

PMID:37723554

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10506217/

Abstract

BACKGROUND

Fungi are a readily available source of naturally generated colored compounds. These compounds might be used as radiosensitizers for treating cancer cells.

METHODS

Aspergillus nidulans was examined for its color-producing ability in Potato dextrose agar (PDA) broth medium. The pigment was characterized by Ultraviolet (UV) spectrophotometer and Gas Chromatography Mass Spectrometry (GC/MS). Pigment extracts from A. nidulans were studied for their cytotoxic effects on the growth of human larynx carcinoma cell line (HEp-2) with or without exposure to γ-radiation at three different doses (5, 10, and 15 Gy). A. nidulans pigment cytotoxic activity was tested against normal Vero cells. Cell apoptosis was studied using flow cytometry. Gene expression of P53, Caspase 3 and Bcl-2 were quantified.

RESULTS

Ultraviolet spectrum and GC/MS revealed the ability of Aspergillus nidulans to produce Rhodopin pigment. HEp-2 cells treated with A. nidulans pigment only give IC about 208 µg/ml. In contrast, when treated with the pigment +10 Gy γ-radiation, it give about 115 µg/ml. However, for normal cells, lower cytotoxic activity was detected. Treatment with pigment (208 g/mL) caused about 50% ± 1.0 total apoptosis level and gene expression of P53: 2.3 fold and Caspase 3: 1.84 fold in respect to untreated HEp-2), while Bcl-2 was decreased (Bcl-2: 0.63 fold in respect to untreated HEp-2). Furthermore, treated with pigment (115 µg/mL) + 10Gy caused about 47.41% ± 1.7 total apoptosis level and P53: 2.53 fold and Caspase 3: 2.0 fold in respect to untreated HEp-2, while Bcl-2 was downregulated (Bcl-2: 0.61 fold in respect to untreated HEp-2).

CONCLUSION

This study concluded that the anti-cancer activity of Aspergillus nidulans pigment was enhanced by ionizing radiation at 10 Gy, as well as its low cytotoxic activity against normal Vero cells.

摘要

背景

真菌是天然生成有色化合物的易得来源。这些化合物可能被用作治疗癌细胞的放射增敏剂。

方法

在马铃薯葡萄糖琼脂（PDA）肉汤培养基中检查了aspergillus nidulans 产生颜色的能力。用紫外线（UV）分光光度计和气相色谱-质谱联用（GC/MS）对色素进行了表征。研究了来自aspergillus nidulans 的色素提取物在不同剂量（5、10 和 15 Gy）的γ辐射暴露下对人喉癌细胞系（HEp-2）生长的细胞毒性作用。用aspergillus nidulans 色素处理正常 vero 细胞，测试其细胞毒性活性。用流式细胞术研究细胞凋亡。定量测定 P53、Caspase 3 和 Bcl-2 的基因表达。

结果

紫外线光谱和 GC/MS 显示aspergillus nidulans 具有产生罗地奥平色素的能力。仅用 Aspergillus nidulans 色素处理的 Hep-2 细胞的 IC 约为 208μg/ml。相比之下，当用色素+10 Gy 伽马射线处理时，它的 IC 约为 115μg/ml。然而，对于正常细胞，检测到较低的细胞毒性活性。用色素（208μg/ml）处理约引起 50%±1.0 的总凋亡水平和 P53 的基因表达：未处理的 Hep-2 的 2.3 倍，Caspase 3：1.84 倍，而 Bcl-2 降低（Bcl-2：未处理的 Hep-2 的 0.63 倍）。此外，用色素（115μg/ml）+10Gy 处理约引起 47.41%±1.7 的总凋亡水平和 P53：未处理的 Hep-2 的 2.53 倍，Caspase 3：2.0 倍，而 Bcl-2 下调（Bcl-2：未处理的 Hep-2 的 0.61 倍）。