Freeman G B, Gibson G E
J Neurochem. 1986 Dec;47(6):1924-31. doi: 10.1111/j.1471-4159.1986.tb13109.x.
The effects of hypoxia on release of endogenous 3,4-dihydroxyphenylethylamine (DA, dopamine) were investigated in mouse striatal slices. Following a 60-min preincubation, potassium increased DA release 12 times between zero and 1 min. By 10 min, uptake processes exceeded release and DA levels in the media decreased. Hypoxia (low oxygen) and anoxia (no oxygen) increased DA in the media by 120 and 205%, respectively, but did not alter dihydroxyphenylacetic acid concentrations. Under similar conditions, anoxia increased [3H]DA uptake eight-fold. For the uptake studies, the amount of DA added to the media was critical; in the presence of high concentrations of DA, anoxia reduced reuptake. Regardless of exogenous DA, hypoxia and anoxia increased extracellular DA, which may play a role in ischemic cell damage.
在小鼠纹状体切片中研究了缺氧对内源性3,4 - 二羟基苯乙胺（DA，多巴胺）释放的影响。经过60分钟的预孵育后，在0至1分钟内，钾使DA释放增加了12倍。到10分钟时，摄取过程超过释放过程，培养基中的DA水平下降。缺氧（低氧）和无氧（无氧）分别使培养基中的DA增加了120%和205%，但未改变二羟基苯乙酸的浓度。在类似条件下，无氧使[3H]DA摄取增加了八倍。对于摄取研究，添加到培养基中的DA量至关重要；在高浓度DA存在下，无氧会降低再摄取。无论有无外源性DA，缺氧和无氧都会增加细胞外DA，这可能在缺血性细胞损伤中起作用。
