McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, United States of America.
Department of Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, PA, United States of America.
Biomed Phys Eng Express. 2023 Oct 4;9(6). doi: 10.1088/2057-1976/acfb05.
Biologic scaffolds are extensively used in various clinical applications such as musculotendinous reconstruction, hernia repair or wound healing. Biologic scaffolds used in these applications vary in species, breed and tissue of origin, and other variables that affect their properties. Decellularization and sterilization processes also determine the characteristics of these scaffolds. The goal of the present study is to compare the composition and mechanical properties of decellularized porcine placental scaffolds from three different porcine breeds: Landrace, York and Duroc. Placental extracellular matrix (ECM) scaffolds from the three porcine breeds preserved the amnion/chorion ECM structure and the basement membrane markers laminin and collagen type IV. ECM placental scaffolds showed similar contents of collagen, elastin and lipids, and minimal differences in glycosaminoglycans content. Mechanical properties from the three breeds ECM placental scaffolds were also similar and stable for 24 months. While this study serves as preliminary characterization of porcine ECM scaffolds, future studies will determine their compatibility and suitability for tissue engineering applications.
生物支架广泛应用于各种临床应用，如肌肉腱重建、疝修补或伤口愈合。用于这些应用的生物支架在物种、品种和组织来源以及影响其特性的其他变量上有所不同。脱细胞和消毒过程也决定了这些支架的特性。本研究的目的是比较三种不同猪品种（长白猪、约克夏和杜洛克）的脱细胞猪胎盘支架的组成和力学性能。胎盘细胞外基质（ECM）支架保留了羊膜/绒毛膜 ECM 结构和基底膜标志物层粘连蛋白和 IV 型胶原。胎盘 ECM 支架的胶原、弹性蛋白和脂质含量相似，糖胺聚糖含量差异极小。三种 ECM 胎盘支架的力学性能也相似，24 个月内保持稳定。虽然本研究是对猪 ECM 支架的初步表征，但未来的研究将确定它们在组织工程应用中的相容性和适用性。
