单细胞测序中的微流控设计及其在癌症精准医疗中的应用。
Microfluidic design in single-cell sequencing and application to cancer precision medicine.
机构信息
CUHK(SZ)-Boyalife Joint Laboratory of Regenerative Medicine Engineering, Biomedical Engineering Programme, School of Medicine, The Chinese University of Hong Kong, Shenzhen 518172, China; Ciechanover Institute of Precision and Regenerative Medicine, School of Medicine, The Chinese University of Hong Kong, Shenzhen 518172, China.
State Key Laboratory of Physical Chemistry of Solid Surfaces, MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, P.R. China; Institute of Molecular Medicine, State Key Laboratory of Oncogenes and Related 12 Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, P.R. China.
出版信息
Cell Rep Methods. 2023 Sep 25;3(9):100591. doi: 10.1016/j.crmeth.2023.100591. Epub 2023 Sep 18.
Single-cell sequencing (SCS) is a crucial tool to reveal the genetic and functional heterogeneity of tumors, providing unique insights into the clonal evolution, microenvironment, drug resistance, and metastatic progression of cancers. Microfluidics is a critical component of many SCS technologies and workflows, conferring advantages in throughput, economy, and automation. Here, we review the current landscape of microfluidic architectures and sequencing techniques for single-cell omics analysis and highlight how these have enabled recent applications in oncology research. We also discuss the challenges and the promise of microfluidics-based single-cell analysis in the future of precision oncology.
单细胞测序（SCS）是揭示肿瘤遗传和功能异质性的重要工具，为癌症的克隆进化、微环境、耐药性和转移进展提供了独特的见解。微流控技术是许多 SCS 技术和工作流程的关键组成部分，在通量、经济性和自动化方面具有优势。在这里，我们回顾了用于单细胞组学分析的微流控架构和测序技术的现状，并强调了它们如何使肿瘤学研究中的最近应用成为可能。我们还讨论了基于微流控的单细胞分析在精准肿瘤学未来面临的挑战和前景。
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