Biswas Arka, Chandel Arvind K Singh, Vadadoriya Nikita, Mamtani Vijay, Jewrajka Suresh K
Membrane Science and Separation Technology Division, Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), G. B. Marg, Bhavnagar, Gujarat 364002, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
ACS Appl Mater Interfaces. 2023 Oct 4;15(39):46333-46346. doi: 10.1021/acsami.3c09743. Epub 2023 Sep 19.
We report the construction of amphiphilic conetwork (APCN)-based surfaces with potent antimicrobial activity and biofilm inhibition ability. The construction strategy is based on the separation of lipophilic alkyl groups (>C6) from the cationic network to obtain good antibacterial properties. The reaction of partially alkylated poly(vinyl imidazole) with the activated halide compounds followed by coating a glass or poly(dimethylsiloxane) (PDMS) sheet leads to the formation of the APCN surface. The dangling alkyl chains, crosslinking junctions, and unreacted vinyl imidazole groups are heterogeneously distributed in the APCNs. The swelling, mechanical property, and phase morphology of the APCN films have been evaluated. Bacterial cell disrupting potency of the APCN coatings increases with increasing alkyl chain length from C6 to C18 with somewhat more of an effect on as compared to bacteria. The minimum inhibitory amount of the APCNs on glass and a hydrophobic PDMS surface is in the range of 0.02-0.04 mg/cm depending on the chain length of the alkyl and the degree of quaternization. The effect of the type of crosslinker for the construction of the conetwork on the antimicrobial property has been evaluated to elucidate the exclusive design of the APCNs. The APCN-based coatings provide potent biocidal activity without much negatively affecting the hemocompatibility and cytocompatibility. These APCNs provide a good model system for comparative evaluation of the biocidal property and structural effect on the biocidal activity.
我们报道了具有强大抗菌活性和生物膜抑制能力的两亲性互穿网络（APCN）基表面的构建。构建策略基于将亲脂性烷基（>C6）与阳离子网络分离以获得良好的抗菌性能。部分烷基化的聚（乙烯基咪唑）与活性卤化物化合物反应，然后涂覆在玻璃或聚（二甲基硅氧烷）（PDMS）片材上，导致形成APCN表面。悬垂的烷基链、交联节点和未反应的乙烯基咪唑基团在APCNs中呈非均匀分布。对APCN薄膜的溶胀、机械性能和相形态进行了评估。APCN涂层对细菌细胞的破坏能力随着烷基链长度从C6增加到C18而增强，与 细菌相比，对 细菌的影响更大。根据烷基链长度和季铵化程度，APCNs在玻璃和疏水性PDMS表面上的最小抑制量在0.02 - 0.04 mg/cm范围内。评估了用于构建互穿网络的交联剂类型对抗菌性能的影响，以阐明APCNs的独特设计。基于APCN的涂层具有强大的杀菌活性，而对血液相容性和细胞相容性没有太大的负面影响。这些APCNs为比较评估杀菌性能和杀菌活性的结构效应提供了一个良好的模型系统。
