Department of Dermatology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China.
Department of Dermatology, The People's Hospital of Chongzhou, Chengdu, Sichuan, China.
Amino Acids. 2023 Oct;55(10):1417-1428. doi: 10.1007/s00726-023-03320-2. Epub 2023 Sep 19.
Acne vulgaris is a chronic inflammatory disease with high incidence, diverse clinical manifestations, poor clinical efficacy, and easy recurrence. Recent studies have found that the occurrence of acne is related to metabolic factors such as insulin resistance; however, the specific mechanism of action remains unclear. This study aimed to identify significantly different metabolites and related metabolic pathways in the serum of acne vulgaris patients with or without insulin resistance. LC-MS/MS was used to analyze serum samples from patients about acne with insulin resistance (n = 51) and acne without insulin resistance (n = 69) to identify significant metabolites and metabolic pathways. In this study, 18 significant differential metabolites were screened for the first time. In the positive-ion mode, the upregulated substances were creatine, sarcosine, D-proline, uracil, Phe-Phe, L-pipecolic acid, and DL-phenylalanine; the downregulated substances were tridecanoic acid (tridecylic acid), L-lysine, cyclohexylamine, sphingomyelin (d18:1/18:0), gamma-L-Glu-epsilon-L-Lys, and 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine. In the negative-ion mode, the upregulated substance was cholesterol sulfate, and the downregulated substances were D(-)-beta-hydroxybutyric acid, myristic acid, D-galacturonic acid, and dihydrothymine. Cholesterol sulfate showed the most significant expression among all differential metabolites (VIP = 7.3411). Based on the KEGG database, necroptosis and ABC transporters were the most significantly enriched metabolic pathways in this experiment. The differential metabolites and pathways identified in this study may provide new possibilities for the clinical diagnosis and development of targeted drugs for acne patients with insulin resistance.
寻常痤疮是一种发病率高、临床表现多样、临床疗效差、易复发的慢性炎症性疾病。最近的研究发现，痤疮的发生与胰岛素抵抗等代谢因素有关，但具体作用机制尚不清楚。本研究旨在鉴定有或无胰岛素抵抗寻常痤疮患者血清中的差异代谢物及其相关代谢通路。采用 LC-MS/MS 分析 51 例有胰岛素抵抗寻常痤疮患者和 69 例无胰岛素抵抗寻常痤疮患者的血清样本，以鉴定差异代谢物和代谢通路。本研究首次筛选出 18 种差异代谢物。在正离子模式下，上调物质为肌酸、肌氨酸、D-脯氨酸、尿嘧啶、苯丙氨酸-苯丙氨酸、L-哌啶酸、DL-苯丙氨酸；下调物质为十三烷酸（十三酸）、L-赖氨酸、环己胺、神经鞘磷脂（d18:1/18:0）、γ-L-Glu-epsilon-L-Lys、1,2-二油酰基-sn-甘油-3-磷酸胆碱。在负离子模式下，上调物质为胆固醇硫酸酯，下调物质为 D(-)-β-羟基丁酸、肉豆蔻酸、D-半乳糖醛酸、二氢胸腺嘧啶。在所有差异代谢物中，胆固醇硫酸酯的表达最为显著（VIP=7.3411）。基于 KEGG 数据库，本实验中坏死性凋亡和 ABC 转运体是最显著富集的代谢通路。本研究中鉴定的差异代谢物和通路可能为有胰岛素抵抗寻常痤疮患者的临床诊断和靶向药物开发提供新的可能性。
