Zhu Qingdong, Han Yading, Yang Wentao, Zhu Hang, Li Guangtong, Xu Ke, Long Mingxin
School of Biological Sciences, Jining Medical University, Rizhao, China.
Front Genet. 2023 Sep 1;14:1186192. doi: 10.3389/fgene.2023.1186192. eCollection 2023.
The gene plays a key role in environmental stress response. However, limited studies exist regarding the tomato gene. In this study, we identified 35 genes in tomato by genome-wide identification. Among the 12 chromosomes of tomato, gene is distributed on 10 chromosomes, among which the 7th and 10th chromosomes have no family members, while the 11th chromosome has the most members with 8 family members. Members of this gene family are characterized by long coding sequences, few amino acids, and introns that make up a large proportion of the genetic structure of most members of this family. Moreover, the molecular weight of the proteins of the family members was similar, and the basic proteins were mostly, and the overall distribution was relatively close to neutral (pI = 7). This may indicate that proteins in this family have a more conserved function. In addition, a total of four classes of cis-acting elements were detected in all 35 promoter regions, most of which were associated with biotic and abiotic stresses. The results indicate that gene had a certain response to cold stress, salt stress, ABA treatment and PEG stress. This study provides a new candidate gene for improving tomato stress resistance.
该基因在环境胁迫响应中起关键作用。然而，关于番茄该基因的研究有限。在本研究中，我们通过全基因组鉴定在番茄中鉴定出35个该基因。在番茄的12条染色体中，该基因分布在10条染色体上，其中第7和第10号染色体没有家族成员，而第11号染色体家族成员最多，有8个。该基因家族成员的特点是编码序列长、氨基酸少，且内含子在该家族大多数成员的遗传结构中占很大比例。此外，家族成员蛋白质的分子量相似，碱性蛋白质居多，总体分布相对接近中性（pI = 7）。这可能表明该家族中的蛋白质具有更保守的功能。此外，在所有35个该基因启动子区域共检测到四类顺式作用元件，其中大部分与生物和非生物胁迫相关。结果表明该基因对冷胁迫、盐胁迫、ABA处理和PEG胁迫有一定响应。本研究为提高番茄抗逆性提供了一个新的候选基因。
