Lebedeva Elena I, Babenka Andrei S, Hastemir Pelin, Shchastniy Anatoliy T, Zinovkin Dmitry A, Pranjol Md Zahidul Islam
Department of Histology, Vitebsk State Medical University. Vitebsk, Belarus .
Department of Bioorganic Chemistry. Belarussian State Medical University, Minsk, Belarus.
Int J Mol Cell Med. 2022;11(4):274-284. doi: 10.22088/IJMCM.BUMS.11.4.274.
In this study, we hypothesize that angiogenesis of special hepatic vessels such as sinusoid capillaries or veins is closely associated with increasing production of connective tissue in fibrogenesis. Thirty-six male Wistar rats were induced with hepatitis and cirrhosis of the liver using thioacetamide. The number of sinusoidal capillaries, veins, arteries and the area of connective tissue were counted and determined. Immunohistochemical study was performed on paraffin sections using monoclonal mouse anti-CD31. mRNA expression was determined using qPCR. We found a statistically significant reduction in the number of sinusoidal capillaries (p<0.0001) and an increase in the number of interlobular veins (p<0.0001) in the fibrosis and cirrhosis groups compared to the control group. There are no differences in the number of interlobular arteries (p=0.282) in the three groups. In our analysis, we found that the expression (mRNA) of Fn14 correlated with the number of veins in liver fibrosis (r=0.44, p=0.008). Our data shows that modulation of veins angiogenesis during fibrosis in chronic liver diseases may play an important role in increasing pathological changes of the liver.
在本研究中，我们假设肝血窦毛细血管或静脉等特殊肝血管的血管生成与肝纤维化过程中结缔组织生成增加密切相关。采用硫代乙酰胺诱导36只雄性Wistar大鼠发生肝炎和肝硬化。对肝血窦毛细血管、静脉、动脉的数量以及结缔组织面积进行计数和测定。使用小鼠抗CD31单克隆抗体对石蜡切片进行免疫组织化学研究。采用qPCR测定mRNA表达。我们发现，与对照组相比，纤维化和肝硬化组的肝血窦毛细血管数量有统计学意义的减少（p<0.0001），小叶间静脉数量增加（p<0.0001）。三组间小叶间动脉数量无差异（p=0.282）。在我们的分析中，我们发现Fn14的表达（mRNA）与肝纤维化中的静脉数量相关（r=0.44，p=0.008）。我们的数据表明，慢性肝病纤维化过程中静脉血管生成的调节可能在肝脏病理变化增加中起重要作用。
