Department of General Surgery at Beijing Jishuitan Hospital, The Fourth Clinical Medical College of Peking University, Beijing, China.
Curr Gene Ther. 2023;23(5):391-399. doi: 10.2174/1566523223666230529142423.
Gastric cancer is a well-known malignant tumor that causes millions of deaths worldwide every year. Due to the lack of a specific biomarker for gastric cancer, most patients are diagnosed at an advanced stage of the disease which results in a poor prognosis and a higher death rate. Therefore, novel biomarkers are urgently needed for early diagnosis and to improve the survival rate.
In this study, we conducted RNA sequencing of tumor samples from 21 patients with gastric cancer. A total of 3192 differentially expressed genes (1589 up-regulated and 1603 down-regulated) were identified. Subsequently, we applied a text-mining algorithm for further analysis of these data and selected 30 representative genes to investigate as candidates for novel biomarkers in gastric cancer.
Among these genes, we confirmed transient receptor potential melastatin 8 channels (TRPM8) as a novel biomarker based on Western blot and immunochemistry validation performed on 134 samples. Compared to normal gastric tissue, the tumor tissues exhibited a significantly higher expression level of TRPM8.
This study provides insights into the underlying role of TRPM8 in cell proliferation. In addition, TRPM8 may be used as a potential therapeutic target for patients with gastric cancer.
胃癌是一种众所周知的恶性肿瘤，每年在全球导致数百万人死亡。由于缺乏胃癌的特定生物标志物，大多数患者在疾病晚期被诊断出来，导致预后不良和死亡率较高。因此，迫切需要新的生物标志物来进行早期诊断并提高生存率。
本研究对 21 例胃癌患者的肿瘤样本进行了 RNA 测序。共鉴定出 3192 个差异表达基因（1589 个上调和 1603 个下调）。随后，我们应用文本挖掘算法对这些数据进行了进一步分析，并选择了 30 个代表性基因作为胃癌新生物标志物的候选基因进行研究。
在这些基因中，我们通过对 134 个样本进行 Western blot 和免疫组织化学验证，证实瞬时受体电位 melastatin 8 通道（TRPM8）是一种新型生物标志物。与正常胃组织相比，肿瘤组织中 TRPM8 的表达水平明显更高。
本研究深入了解了 TRPM8 在细胞增殖中的潜在作用。此外，TRPM8 可能成为胃癌患者潜在的治疗靶点。
