Zayed Mervat F, Abdel-Monem Yasser K, Arafa Abeer A, Eisa Wael H
Chemistry Department, Faculty of Science, Menoufia University, Shibin El Kom, Egypt.
Spectroscopy Department, Physics Research Institute, National Research Centre (NRC), Cairo, Egypt.
Microsc Res Tech. 2024 Jan;87(1):149-158. doi: 10.1002/jemt.24419. Epub 2023 Sep 20.
Phytochemical-conjugated silver nanoparticles (AgNPs) are believed to act as a bridge between nanotechnology and therapy. There is a significant need for green and mass production of such materials due to their extensive applications, especially in the biomedical sector. In this study, morin-stabilized silver nanoparticles (morin/AgNPs) were synthesized on a massive scale using a one-pot solid-state technique. The reaction is achieved by ball milling of morin and silver nitrate powders at ambient temperature without any solvent or toxic reagent. The prepared morin/AgNPs exhibited a semi-hexagonal shape and ranged in size from 21 to 43 nm. The x-ray diffraction results elucidated the formation of highly crystalline AgNPs. Fourier transform infrared and x-ray photoelectron spectroscopic analyses prove that the hydroxyl, carbonyl, and aromatic functionalities in morin are playing major roles in the reduction and stabilization of AgNPs. The antioxidant potential of morin/AgNPs was evaluated utilizing 2,2-Diphenyl-1-picryl-hydrazyl (DPPH) assay. Morin/AgNPs exhibited better free radical scavenging activity (IC = 11.7 μg/mL) than morin (IC = 14.8 μg/mL). Furthermore, the synthesized AgNPs showed promising antimicrobial activity against Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, Streptococcus mutans, and Candida albicans. The largest inhibition zones were observed against S. aureus (21.2 ± 0.6 mm) and K. pneumonia (20.3 ± 0.5 mm) bacteria. The foregoing results highlighted the prospective application of morin/AgNPs as a promising antioxidant and antimicrobial material for safe medical applications. RESEARCH HIGHLIGHTS: A simple green route for the large-scale production of AgNPs was developed. Morin acts as reducing/stabilizing agent in solid-state synthesis of AgNPs. Morin/AgNPs exhibited promising antimicrobial and antioxidant activity.
植物化学共轭银纳米颗粒（AgNPs）被认为是纳米技术与治疗之间的桥梁。由于其广泛的应用，特别是在生物医学领域，对这类材料的绿色大规模生产有很大需求。在本研究中，使用一锅固态技术大规模合成了桑色素稳定的银纳米颗粒（桑色素/AgNPs）。该反应通过在室温下对桑色素和硝酸银粉末进行球磨来实现，无需任何溶剂或有毒试剂。制备的桑色素/AgNPs呈现半六边形形状，尺寸范围为21至43纳米。X射线衍射结果阐明了高度结晶的AgNPs的形成。傅里叶变换红外光谱和X射线光电子能谱分析证明，桑色素中的羟基、羰基和芳香官能团在AgNPs的还原和稳定中起主要作用。利用2,2-二苯基-1-苦基肼（DPPH）测定法评估了桑色素/AgNPs的抗氧化潜力。桑色素/AgNPs表现出比桑色素（IC = 14.8μg/mL）更好的自由基清除活性（IC = 11.7μg/mL）。此外，合成的AgNPs对大肠杆菌、肺炎克雷伯菌、金黄色葡萄球菌、变形链球菌和白色念珠菌显示出有前景的抗菌活性。观察到对金黄色葡萄球菌（21.2±0.6毫米）和肺炎克雷伯菌（20.3±0.5毫米）的抑菌圈最大。上述结果突出了桑色素/AgNPs作为一种有前景的抗氧化和抗菌材料在安全医疗应用中的潜在应用。研究亮点：开发了一种简单的绿色路线用于大规模生产AgNPs。桑色素在AgNPs的固态合成中充当还原剂/稳定剂。桑色素/AgNPs表现出有前景的抗菌和抗氧化活性。
