Legan Andrew Wesley, Mehl Hillary L, Varaksa Alex, Callicott Kenneth A
USDA-ARS, Arid Land Agricultural Research Center, Tucson, Arizona, USA.
Microbiol Resour Announc. 2023 Oct 19;12(10):e0052723. doi: 10.1128/MRA.00527-23. Epub 2023 Sep 20.
Toxic molds in the genus synthesize carcinogenic aflatoxins which contaminate crops. The widely applied biocontrol isolate AF36 (NRRL 18543) has a high-quality public genome but lacks corresponding gene annotations. We generated high-quality gene predictions for this isolate by using long-read Nanopore PCR-cDNA sequencing.
该属中的有毒霉菌会合成致癌的黄曲霉毒素,污染农作物。广泛应用的生物防治菌株AF36(NRRL 18543)拥有高质量的公开基因组,但缺乏相应的基因注释。我们通过使用长读长纳米孔PCR-cDNA测序为该菌株生成了高质量的基因预测。