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生物防治菌株AF36(NRRL 18543)的纳米孔PCR-cDNA测序为基因注释提供了信息。

Nanopore PCR-cDNA sequencing of the biocontrol isolate AF36 (NRRL 18543) informs gene annotation.

作者信息

Legan Andrew Wesley, Mehl Hillary L, Varaksa Alex, Callicott Kenneth A

机构信息

USDA-ARS, Arid Land Agricultural Research Center, Tucson, Arizona, USA.

出版信息

Microbiol Resour Announc. 2023 Oct 19;12(10):e0052723. doi: 10.1128/MRA.00527-23. Epub 2023 Sep 20.

DOI:10.1128/MRA.00527-23
PMID:37728564
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10586124/
Abstract

Toxic molds in the genus synthesize carcinogenic aflatoxins which contaminate crops. The widely applied biocontrol isolate AF36 (NRRL 18543) has a high-quality public genome but lacks corresponding gene annotations. We generated high-quality gene predictions for this isolate by using long-read Nanopore PCR-cDNA sequencing.

摘要

该属中的有毒霉菌会合成致癌的黄曲霉毒素,污染农作物。广泛应用的生物防治菌株AF36(NRRL 18543)拥有高质量的公开基因组,但缺乏相应的基因注释。我们通过使用长读长纳米孔PCR-cDNA测序为该菌株生成了高质量的基因预测。

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