Functional Portrait and Genomic Feature of Carbapenem-Resistant Harboring and in China.

The purpose of this research was to analyze the functional portraits and genomic features of carbapenem-resistant carrying and . The resistance mechanism of the strain was verified by experiments. Genomic data were aligned and analyzed in the NCBI database. Growth curve measurements were used to describe the growth characteristics of the bacteria. The virulence of strain was analyzed by serum killing assay and biofilm formation assay. Plasmid conjugation experiments were performed to verify the transferability of plasmids carrying drug-resistance genes. The strain was highly resistant to carbapenems. In addition, ST typing is unknown and has been submitted to Genebank. The strain carried two carbapenemase genes, including and . Among them, was located on a 5.62832 Mb chromosome, and was located on a 172.851 Kb transferable plasmid, which was a very close relative of pIMP-NY7610 in China. The strain also had a variety of virulence genes, which were expressed in the siderophore, capsule, pilus, alginate, flagella, etc. The study suggests that the functional portrait and genomic features of carbapenem-resistant harboring and are unique to China. This outcome represents antibiotic resistance exhibited in the genus by acquiring chromosomes and plasmid genes. The monitoring and supervision of antimicrobial usage must be strengthened since the multi-drug-resistant and moderately virulent will attract much attention in the near future.