Falco Aura, Mondragón-Quiguanas Alejandra, Burbano Laura, Villaquirán-Muriel Miguel Ángel, Correa Adriana, Aranaga Carlos
Departamento de Biología, Facultad de Ciencias, Universidad del Tolima, Ibagué 730006, Colombia.
Grupo de Investigación en Microbiología, Industria y Ambiente (GIMIA), Facultad de Ciencias Básicas, Universidad Santiago de Cali, Cali 760035, Colombia.
Antibiotics (Basel). 2025 Aug 1;14(8):781. doi: 10.3390/antibiotics14080781.
: Antimicrobial Resistance (AMR) is a multifaceted issue that the World Health Organization (WHO) identifies as one of the primary threats to global health for humans, animals, and the environment. In Colombia, AMR has been extensively studied at the hospital level; however, there are limited environmental studies, particularly concerning leachates from landfills. The objective of this study was to identify and determine the genetic relationships, as well as the sensitivity profiles to antibiotics and heavy metals, of ten isolates from a leachate pond. : Identification was conducted using MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight), while genotyping was performed via rep-PCR. Antibiotic susceptibility to β-lactams, aminoglycosides, and quinolones was assessed using the Kirby-Bauer method. Additionally, sensitivity profile to heavy metals was evaluated using the broth microdilution technique. : Rep-PCR analysis indicated that 60% ( = 6/10) of the isolates exhibited a clonal relationship. Sensitivity testing revealed that 30% ( = 3/10) of the isolates displayed reduced sensitivity to aminoglycosides and β-lactams. Finally, the broth microdilution showed that 90% ( = 9/10) of the isolates were tolerant to copper sulfate. : These results provide evidence that landfill leachates may serve as a potential reservoir for bacteria harboring antimicrobial resistance determinants.
抗菌耐药性（AMR）是一个多方面的问题，世界卫生组织（WHO）将其确定为对人类、动物和环境的全球健康的主要威胁之一。在哥伦比亚，已在医院层面广泛研究了AMR；然而，环境研究有限，特别是关于垃圾渗滤液的研究。本研究的目的是鉴定和确定来自渗滤液池的10株分离株的遗传关系，以及对抗生素和重金属的敏感性概况。使用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF）进行鉴定，同时通过重复聚合酶链反应（rep-PCR）进行基因分型。使用 Kirby-Bauer 方法评估对β-内酰胺类、氨基糖苷类和喹诺酮类抗生素的敏感性。此外，使用肉汤微量稀释技术评估对重金属的敏感性概况。重复聚合酶链反应分析表明，60%（=6/10）的分离株表现出克隆关系。敏感性测试显示，30%（=3/10）的分离株对氨基糖苷类和β-内酰胺类抗生素的敏感性降低。最后，肉汤微量稀释显示，90%（=9/十）的分离株对硫酸铜具有耐受性。这些结果证明，垃圾渗滤液可能是携带抗菌耐药性决定因素的细菌的潜在储存库。
