Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.
Infection Control Unit, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.
Emerg Microbes Infect. 2021 Dec;10(1):442-449. doi: 10.1080/22221751.2021.1894903.
IMP-45, a variant of IMP-9, is one of the dominant metallo-β-lactamases (MBLs) in clinical carbapenem-resistant (CRPA) isolates in China. The aim of this study was to investigate the distribution and mechanism of dissemination of . MBL genes were detected by PCR in 173 non-duplicate CRPA isolates collected from Hospital HS in Shanghai and 605 isolates from a multicenter surveillance of in China. In total, 17 IMP-45-producers (14 from Hospital HS and 3 from other hospitals) were identified. Molecular typing identified an outbreak of 11 IMP-45-producing ST508 CRPA in the ICU of Hospital HS. Conjugation assays and whole genome sequencing were conducted among IMP-45-producers. Genomic comparison revealed that 16 -carrying plasmids (9 from this study and 7 from GenBank) shared a similar backbone with IncP-2 -carrying plasmid pOZ176 but lacked -- region. gene was presented in pOZ176, -carrying plasmids (17 from this study and 7 from GenBank) and 15 megaplasmids from GenBank. Phylogenetic analysis of showed that most -carrying plasmids were clustered into a sublineage separate from the one containing pOZ176. This IncP-2 plasmid sublineage contributed to the dissemination of among genetically diverse and recruited multiple resistance genes during its evolution.
IMP-45 是 IMP-9 的一个变体，是中国临床耐碳青霉烯类肺炎克雷伯菌（CRPA）分离株中主要的金属β-内酰胺酶（MBLs）之一。本研究旨在调查. MBL 基因在上海 HS 医院 173 例非重复 CRPA 分离株和中国多中心. 监测中 605 例分离株中通过 PCR 进行检测。共鉴定出 17 株 IMP-45 产生菌（14 株来自 HS 医院，3 株来自其他医院）。分子分型鉴定出 HS 医院 ICU 中发生的 11 株 IMP-45 产生 ST508 CRPA 的暴发。在 IMP-45 产生菌之间进行了接合试验和全基因组测序。基因组比较显示，16 个携带. 基因的质粒（9 个来自本研究，7 个来自 GenBank）与携带 IncP-2 的质粒 pOZ176 具有相似的骨架，但缺乏 -- 区。pOZ176 中存在. 基因，携带. 基因的质粒（17 个来自本研究，7 个来自 GenBank）和 15 个来自 GenBank 的大型质粒。. 基因的系统发育分析表明，大多数携带. 基因的质粒聚集在与包含 pOZ176 的亚谱系分开的一个亚谱系中。这个 IncP-2 质粒亚谱系促进了. 基因在遗传上多样化的菌株之间的传播，并在其进化过程中招募了多个耐药基因。
