Central Research Laboratory, Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, India.
Department of Microbiology and Biotechnology, Presidency College (Autonomous), Chennai, India.
Microb Drug Resist. 2023 Nov;29(11):504-509. doi: 10.1089/mdr.2023.0040. Epub 2023 Sep 20.
Infections caused by carbapenem-resistant (CRKP) are a major threat to public health. Timely detection of CRKP will help treat patients with appropriate antibiotics. This study aimed to evaluate the performance of the carbapenemase Nordmann-Poirel (CarbaNP), modified carbapenem inactivation (mCIM), and EDTA carbapenem inactivation (eCIM) methods for the detection of CRKP. We compared the results of the three assays with that of real-time PCR. In total, 195 isolates, including 150 carbapenem-resistant and 45 carbapenem-susceptible isolates, were investigated. Carbapenem-resistance genes, such as , , , , and , were identified using real-time PCR. Among the 150 CRKP isolates, 94 (62.7%) were positive for , 29 (19.3%) were positive for , and 27 (18%) were positive for both and . For detecting CRKP isolates, CarbaNP, mCIM, and eCIM showed 96.0%, 95.4%, and 96.7% sensitivity, respectively, and all three methods showed 100% specificity. All three phenotypic confirmatory tests are reliable for identifying CRKP, easy to perform, cost-effective, and can be incorporated with routine antibiotic susceptibility testing.
耐碳青霉烯肠杆菌科细菌（CRKP）引起的感染对公共卫生构成重大威胁。及时发现 CRKP 将有助于患者使用适当的抗生素进行治疗。本研究旨在评估碳青霉烯酶诺德曼-波莱尔（CarbaNP）、改良碳青霉烯失活（mCIM）和 EDTA 碳青霉烯失活（eCIM）方法检测 CRKP 的性能。我们将这三种检测方法的结果与实时 PCR 的结果进行了比较。共检测了 195 株分离株，包括 150 株耐碳青霉烯和 45 株碳青霉烯敏感株。使用实时 PCR 鉴定了碳青霉烯耐药基因，如 、 、 、 、 。在 150 株 CRKP 分离株中，94 株（62.7%）对 阳性，29 株（19.3%）对 阳性，27 株（18%）对 阳性。检测 CRKP 分离株时，CarbaNP、mCIM 和 eCIM 的灵敏度分别为 96.0%、95.4%和 96.7%，所有三种方法的特异性均为 100%。三种表型确证试验均可靠地鉴定 CRKP，易于操作，具有成本效益，可与常规抗生素敏感性试验相结合。
