Universidade de São Paulo - USP, Bauru School of Dentistry, Bauru, SP, Brazil.
University of Bern, Department of Preventive, Restorative and Pediatric Dentistry, Bern, Switzerland.
Braz Oral Res. 2023 Sep 15;37:e085. doi: 10.1590/1807-3107bor-2023.vol37.0085. eCollection 2023.
The objective of this study was to compare the protein profile of the acquired enamel pellicle (AEP) formed in vivo in patients with or without gastroesophageal reflux disease (GERD), and with or without erosive tooth wear (ETW). Twenty-four volunteers were divided into 3 groups: 1) GERD and ETW; 2) GERD without ETW; and 3) control (without GERD). The AEP formed 120 min after prophylaxis was collected from the lingual/palatal surfaces. The samples were subjected to mass spectrometry (nLC-ESI-MS/MS) and label-free quantification by Protein Lynx Global Service software. A total of 213 proteins were identified, or 119, 92 and 106 from each group, respectively. Group 2 showed a high number of phosphorylated and calcium-binding proteins. Twenty-three proteins were found in all the groups, including 14-3-3 protein zeta/delta and 1-phosphatidylinositol. Several intracellular proteins that join saliva after the exfoliation of oral mucosa cells might have the potential to bind hydroxyapatite, or participate in forming supramolecular aggregates that bind to precursor proteins in the AEP. Proteins might play a central role in protecting the dental surface against acid dissolution.
本研究旨在比较伴有或不伴有胃食管反流病（GERD）和/或酸蚀性牙齿磨损（ETW）的患者体内形成的获得性牙面涂层（AEP）的蛋白质谱。将 24 名志愿者分为 3 组：1）GERD 和 ETW；2）GERD 无 ETW；3）对照组（无 GERD）。在预防措施后 120 分钟，从舌/腭表面采集 AEP。使用纳升液相色谱-电喷雾串联质谱（nLC-ESI-MS/MS）和 Protein Lynx Global Service 软件进行非标记定量分析。共鉴定出 213 种蛋白质，分别来自每组的 119、92 和 106 种蛋白质。组 2 显示出大量磷酸化和钙结合蛋白。在所有组中均发现了 23 种蛋白质，包括 14-3-3 蛋白 zeta/delta 和 1-磷酸肌醇。一些在口腔黏膜细胞脱落后进入唾液的细胞内蛋白可能具有结合羟磷灰石的潜力，或参与形成超分子聚集体，与 AEP 中的前体蛋白结合。蛋白可能在保护牙面免受酸溶解方面发挥核心作用。
