College of Environmental Science and Engineering, Guilin University of Technology, Guilin, People's Republic of China.
Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin, People's Republic of China.
Environ Technol. 2024 Sep;45(22):4550-4564. doi: 10.1080/09593330.2023.2259091. Epub 2023 Sep 20.
P-bromophenol (4-BP) is a toxic halogenated phenolic organic compound. The conventional treatment processes for 4-BP elimination are costly and inefficient, with complete mineralization remaining a challenge for water treatment. To overcome these limitations, we investigated the treatment of 4-BP in a membrane biofilm reactor (MBfR) using hydrogen as an electron donor. The pathway of 4-BP degradation within the H-MBfR was investigated through long-term operational experiments by considering the effect of nitrate and 4-BP concentrations, hydrogen partial pressure, static experiments, and microbial community diversity, which was studied using 16S rRNA. The results showed that H-MBfR could quickly remove approximately 100% of 4-BP (up to 20 mg/L), with minimal intermediate product accumulation and 10 mg/L of nitrate continuously reduced. The microbial community structure showed that the presence of H created an anaerobic environment, and was the dominant functional genus involved in the degradation of 4-BP. The genes encoding related enzymes were further enhanced. This study provides an economically viable and environmentally friendly bioremediation technique for water bodies that contain 4-BP and nitrates.
对溴苯酚（4-BP）是一种有毒的卤代酚类有机化合物。传统的 4-BP 消除处理工艺成本高且效率低，完全矿化仍是水处理的一个挑战。为了克服这些限制，我们研究了在膜生物膜反应器（MBfR）中使用氢气作为电子供体处理 4-BP。通过考虑硝酸盐和 4-BP 浓度、氢气分压、静态实验和微生物群落多样性的影响的长期运行实验，研究了 H-MBfR 中 4-BP 降解的途径，使用 16S rRNA 研究了微生物群落多样性。结果表明，H-MBfR 可以快速去除约 100%的 4-BP（高达 20mg/L），中间产物积累最少，10mg/L 的硝酸盐持续减少。微生物群落结构表明，H 的存在创造了一个厌氧环境，是参与 4-BP 降解的主要功能属。编码相关酶的基因进一步增强。本研究为含有 4-BP 和硝酸盐的水体提供了一种经济可行且环保的生物修复技术。
