Institute of Basic Medicine and Forensic Medicine, North Sichuan Medical College, Nanchong 637000, Sichuan, China.
Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang 641112, China.
Res Vet Sci. 2023 Nov;164:105019. doi: 10.1016/j.rvsc.2023.105019. Epub 2023 Sep 14.
The aim of this study is to explore the effects of fluoride on the innate immunity, intestinal mechanical barrier, and immune barrier of C57BL/6 mice, as well as to analyze the degree of structural and tissue damage, providing reference data for related research. Mice were randomly divided into four groups and then treated with 0 mg/L (control), 50 mg/L, 100 mg/L, 125 mg/L sodium fluoride solution, respectively, for 120 days. Histological technique, ELISA, MTT colorimetry methods were used to detect and analyze the effects of different concentrations of fluoride on the intestinal morphology, mechanical barrier and the immune functions and innate immunity of mice. The results showed that compared with the control group, the villi were injured in different degrees of the three fluoride groups, the number of goblet cells, the protein expression levels of connexin ZO-1, Claudin-1 and Occludin, the content of Diamine Oxidase (DAO), endotoxin (ET) and D-lactic acid (D-LA), the activity of natural killer cell (NK cells), the number and percentage of neutrophils and erythrocytes, the phagocytic rate of neutrophils, and the rate of C3bR rosette (which is formed by the adhesion of C3b receptors on the red blood cell membrane to complement sensitized yeast) and IC rosette (which is formed by the adhesion of C3b molecules in the immunecomplex adhered to the red blood cell membrane to non sensitized yeast) of red blood cells, the content of interlenkin 1 beta (IL-1β) and interlenkin 8 (IL-8), the number and percentage of lymphocytes decreased with the increasing of fluoride concentration. In addition, the content of the Immunoglobulin A (sIgA) showed a trend of increase at first and then decrease in salivary gland and jejunum. It is concluded that excessive intake of fluoride for a long time has a certain damage effect on the intestinal tract, leading to an increase in the permeability of the intestinal tract, thereby destroying the mechanical and immune barrier function of the intestinal tract.
本研究旨在探讨氟化物对 C57BL/6 小鼠固有免疫、肠道机械屏障和免疫屏障的影响，并分析其结构和组织损伤程度，为相关研究提供参考数据。将小鼠随机分为四组，分别给予 0mg/L（对照组）、50mg/L、100mg/L、125mg/L 氟化钠溶液，连续灌胃 120 天。采用组织学技术、ELISA、MTT 比色法检测分析不同浓度氟化物对小鼠肠道形态、机械屏障和免疫功能及固有免疫的影响。结果显示，与对照组相比，3 个氟化物组的绒毛均有不同程度的损伤，杯状细胞数量减少，紧密连接蛋白 ZO-1、Claudin-1 和 Occludin 的蛋白表达水平降低，二胺氧化酶（DAO）、内毒素（ET）和 D-乳酸（D-LA）含量升高，自然杀伤细胞（NK 细胞）活性降低，中性粒细胞和红细胞数量及中性粒细胞百分比、中性粒细胞吞噬率及红细胞 C3b 受体花环（红细胞膜上 C3b 受体与补体致敏酵母黏附形成）和免疫复合物中 C3b 分子黏附于非致敏酵母形成的免疫复合物花环（IC 花环）率降低，白细胞介素 1β（IL-1β）和白细胞介素 8（IL-8）含量降低，唾液腺和空肠组织中免疫球蛋白 A（sIgA）含量呈先升高后降低的趋势。结论：长期过量摄入氟化物对肠道有一定损伤作用，导致肠道通透性增加，从而破坏肠道的机械和免疫屏障功能。
