College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471000, Henan, PR China.
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471000, Henan, PR China.
Chemosphere. 2019 Nov;234:409-418. doi: 10.1016/j.chemosphere.2019.06.080. Epub 2019 Jun 12.
Intestinal microflora play an important role in maintaining the homeostasis of the intestinal microenvironment, but fluoride-induced changes in intestinal mechanical barrier and intestinal microflora have not been well studied. Given this paucity of information, this study aims to determine the effects of high fluoride level on intestinal mechanical barrier and intestinal microflora in the cecum of mice. Seventy-two female 21-day-old Kunming mice were randomly assigned to three groups and raised for 70 days. Changes in intestinal pathomorphology and intestinal epithelial cell proliferation were observed by haematoxylin and eosin-staining and Brdu measurement, respectively. The distribution of goblet cells, glycoproteins and mast cells was analysed through Alcian blue and periodic acid-Schiff (AB-PAS) staining, Periodic Acid-Schiff (PAS) staining, and toluidine blue staining. Results showed that excessive fluoride damaged the structure of the cecal tissues, inhibited epithelial cell proliferation and decreased the relative distribution of goblet cells, glycoproteins and mast cells that are involved in defense responses. Intestinal microflora sequencing analysis revealed that the composition of the diversity and composition of intestinal microflora was altered by excessive fluoride based on 16S rRNA amplicon sequencing. The relative abundance of Firmicutes (P = 0.03174), Bacteroidetes (P = 0.04462), Actinobacteria (P = 0.01085) and Spirochacteria (P = 0.04084) was significantly changed in the fluoride group as compared with the control group. In conclusion, excessive fluoride intake induced intestinal barrier damage, leading to changes in cecal composition, epithelium secretion and intestinal microflora.
肠道微生物在维持肠道微环境的稳态中发挥着重要作用，但氟化物诱导的肠道机械屏障和肠道微生物变化尚未得到很好的研究。鉴于此信息不足，本研究旨在确定高氟水平对小鼠盲肠肠道机械屏障和肠道微生物的影响。将 72 只 21 日龄雌性昆明小鼠随机分为 3 组，饲养 70 天。通过苏木精-伊红染色和 Brdu 测量分别观察肠道形态学变化和肠上皮细胞增殖。通过阿尔辛蓝和过碘酸希夫（AB-PAS）染色、过碘酸希夫（PAS）染色和甲苯胺蓝染色分析杯状细胞、糖蛋白和肥大细胞的分布。结果表明，过量氟化物破坏了盲肠组织的结构，抑制了上皮细胞的增殖，降低了参与防御反应的杯状细胞、糖蛋白和肥大细胞的相对分布。肠道微生物测序分析显示，基于 16S rRNA 扩增子测序，过量氟化物改变了肠道微生物多样性和组成。与对照组相比，氟化物组厚壁菌门（Firmicutes）（P=0.03174）、拟杆菌门（Bacteroidetes）（P=0.04462）、放线菌门（Actinobacteria）（P=0.01085）和螺旋体门（Spirochaetes）（P=0.04084）的相对丰度显著变化。结论：摄入过量氟化物会导致肠道屏障损伤，从而导致盲肠组成、上皮分泌和肠道微生物发生变化。
