Plant Tissue Culture and Genetic Engineering Lab, Department of Biotechnology, S.A.S. Nagar, Ministry of Science and Technology (Government of India), National Agri-Food Biotechnology Institute (NABI), Sector 81, Knowledge City, Mohali, Punjab, 140306, India.
Department of Biotechnology, Panjab University, Chandigarh, 160014, India.
Mol Biol Rep. 2023 Nov;50(11):9061-9072. doi: 10.1007/s11033-023-08743-4. Epub 2023 Sep 20.
The GHMP kinase gene family encompasses ATP-dependent kinases, significantly involved in the biosynthesis of isoprenes, amino acids, and metabolism of carbohydrates. Banana is a staple tropical crop that is globally consumed but known for high sensitivity to salt, cold, and drought stresses. The GHMP kinases are known to play a significant role during abiotic stresses in plants. The present study emphasizes the role of GHMP kinases in various abiotic stress conditions in banana.
We identified 12 GHMP kinase (MaGHMP kinase) genes in the banana genome database and witnessed the presence of the conserved Pro-X-X-X-Gly-Leu-X-Ser-Ser-Ala domain in their protein sequences. All genes were found to be involved in ATP-binding and carried kinase activity confronting their biological roles in the isoprene (27%) and amino acid (20%) biosyntheses. The expression analysis of genes during cold, drought, and salt stress conditions in tissue culture grown banana cultivar Rasthali plants showed a significant involvement of MaGHMP kinase genes in these stress conditions. The highest expression of MaGHMP kinase3 (8.5 fold) was noted during cold stress, while MaGHMP kinase1 (25 fold and 40.01 fold) showed maximum expression during drought and salt stress conditions in leaf tissue of Rasthali.
Our findings suggested that MaGHMP kinase1 (MaHSK) and MaGHMP kinase3 (MaGlcAK) could be considered promising candidates for thwarting the abiotic stresses in banana.
GHMP 激酶基因家族包括 ATP 依赖性激酶，它们在异戊烯、氨基酸和碳水化合物代谢的生物合成中起着重要作用。香蕉是一种主要的热带作物，在全球范围内被消费，但对盐、冷和干旱胁迫非常敏感。已知 GHMP 激酶在植物的非生物胁迫中起着重要作用。本研究强调了 GHMP 激酶在香蕉各种非生物胁迫条件下的作用。
我们在香蕉基因组数据库中鉴定了 12 个 GHMP 激酶（MaGHMP 激酶）基因，并在它们的蛋白质序列中观察到保守的 Pro-X-X-X-Gly-Leu-X-Ser-Ser-Ala 结构域的存在。所有基因都被发现参与 ATP 结合，并具有激酶活性，这与其在异戊烯（27%）和氨基酸（20%）生物合成中的生物学作用相对应。在组织培养中生长的香蕉栽培品种 Rasthali 植物的冷、干旱和盐胁迫条件下的基因表达分析表明，MaGHMP 激酶基因在这些胁迫条件下有显著的参与。MaGHMP 激酶 3（8.5 倍）在冷胁迫下的表达最高，而 MaGHMP 激酶 1（25 倍和 40.01 倍）在 Rasthali 叶片组织中干旱和盐胁迫条件下的表达最高。
我们的研究结果表明，MaGHMP 激酶 1（MaHSK）和 MaGHMP 激酶 3（MaGlcAK）可以被认为是香蕉抵御非生物胁迫的有前途的候选基因。
