Plant Tissue Culture and Genetic Engineering Lab, Department of Biotechnology, Ministry of Science and Technology (Government of India), National Agri-Food Biotechnology Institute (NABI), Sector 81, Knowledge City, S.A.S. Nagar, Mohali, 140306, Punjab, India.
Department of Biotechnology, Panjab University, Chandigarh, 160014, India.
Appl Biochem Biotechnol. 2022 Nov;194(11):5456-5473. doi: 10.1007/s12010-022-04065-6. Epub 2022 Jul 5.
The TCP transcription factor gene family is highly conserved among the plant species. It plays a major role in the regulation of flower symmetry, cell division, and development of leaf, fibre, and nodule in the plants by controlling the synthesis of various plant hormones. Banana is a major staple crop in the world. However, Fusarium oxysporum f. sp. cubense (Foc) infection is a major threat to banana production. The role of TCP gene family during the Foc infection is not explored till now. Herein, a total of 27 non-redundant TCP (MaTCP) gene sequences were retrieved from the banana genome and analysed for structural characteristics, phylogenetic correlation, subcellular, and chromosomal localizations. Phylogenetic analysis showed that the MaTCP proteins were highly conserved among different species and found to be the closest relative of the Oryza sativa and Zea mays. Promoter analysis of the TCP sequences showed that the cis-acting regulatory elements are associated with various stresses and environmental and hormonal signals. The higher transcript accumulation in developing tissues (fruit finger, leaves, and stem) than of mature tissues (peel and pulp) showed a significant role of MaTCP in banana (cv. Rasthali) growth and development. Further, higher expression of the certain MaTCPs in Foc race 1-infected root (MaTCP2, MaTCP4, MaTCP6) and leaf (MaTCP9 and MaTCP11) tissues of Rasthali indicated their promising role during Fusarium infection. This study will underpin the facet of TCP transcription factors on the development of biotic (Foc) stress resistance in banana.
TCP 转录因子基因家族在植物物种中高度保守。它通过控制各种植物激素的合成，在调控花对称性、细胞分裂以及叶片、纤维和根瘤的发育方面发挥主要作用。香蕉是世界上主要的主食作物之一。然而，尖孢镰刀菌古巴专化型（Foc）的感染是香蕉生产的主要威胁。到目前为止，还没有探索 TCP 基因家族在 Foc 感染过程中的作用。在此，从香蕉基因组中总共检索到 27 个非冗余 TCP（MaTCP）基因序列，并对其结构特征、系统发育相关性、亚细胞和染色体定位进行了分析。系统发育分析表明，MaTCP 蛋白在不同物种之间高度保守，与水稻和玉米的关系最为密切。TCP 序列的启动子分析表明，顺式作用调控元件与各种胁迫以及环境和激素信号有关。在发育组织（果指、叶片和茎）中的转录本积累高于成熟组织（果皮和果肉），表明 MaTCP 在香蕉（Rasthali 品种）生长和发育中发挥重要作用。此外，MaTCP2、MaTCP4、MaTCP6 在 Foc 1 型感染的根（MaTCP2、MaTCP4、MaTCP6）和叶（MaTCP9 和 MaTCP11）组织中的较高表达表明它们在尖孢镰刀菌感染过程中具有重要作用。本研究将为 TCP 转录因子在香蕉生物（Foc）胁迫抗性发育方面提供一个新视角。
