Molina Mariano A, Melchers Willem J G, Andralojc Karolina M, Leenders William P J, Huynen Martijn A
Department of Medical Microbiology, Radboud University Medical Center, 6500 HB, Nijmegen, the Netherlands.
Department of Medical Microbiology, Radboud Institute for Molecular Life Sciences, Nijmegen, the Netherlands.
Comput Struct Biotechnol J. 2023 Sep 13;21:4424-4431. doi: 10.1016/j.csbj.2023.09.011. eCollection 2023.
The cervicovaginal microbiome (CVM) is a dynamic continuous microenvironment that can be clustered in microbial community state types (CSTs) and is associated with women's cervical health. -depleted communities particularly associate with an increased susceptibility for persistence of high-risk human papillomavirus (hrHPV) infections and progression of disease, but the long-term ecological dynamics of CSTs after hrHPV infection diagnosis remain poorly understood. To determine such dynamics, we examined the CVM of our longitudinal cohort of 141 women diagnosed with hrHPV infection at baseline with collected cervical smears at two timepoints six-months apart. Here we describe that the long-term microbiome dissimilarity has a positive correlation with microbial diversity at both visits and that women with high abundance and dominance for at baseline exhibit more similar microbiome composition at second visit than women with -depleted communities at baseline. We further show that the species and associate with CST changes between both visits. Lastly, we also observe that is associated with the stability of -depleted communities while is associated with the instability of -dominated communities. Our data suggest dynamic patterns of cervicovaginal CSTs during hrHPV infection, which could be potentially used to develop microbiome-based therapies against infection progression towards disease.
宫颈阴道微生物群（CVM）是一个动态的连续微环境，可分为微生物群落状态类型（CSTs），并与女性的宫颈健康相关。菌群减少的群落尤其与高危型人乳头瘤病毒（hrHPV）感染持续存在及疾病进展的易感性增加有关，但hrHPV感染诊断后CSTs的长期生态动态仍知之甚少。为了确定这种动态变化，我们检查了141名在基线时被诊断为hrHPV感染的女性纵向队列的CVM，在相隔6个月的两个时间点收集宫颈涂片。在此我们描述，长期微生物群差异与两次检查时的微生物多样性呈正相关，并且在基线时具有高丰度和优势菌的女性在第二次检查时比在基线时菌群减少的女性表现出更相似的微生物群组成。我们进一步表明，物种[具体物种1]和[具体物种2]与两次检查之间的CST变化有关。最后，我们还观察到[具体因素1]与菌群减少的群落稳定性有关，而[具体因素2]与优势菌群落的不稳定性有关。我们的数据表明了hrHPV感染期间宫颈阴道CSTs的动态模式，这可能潜在地用于开发基于微生物群的疗法以对抗感染向疾病的进展。
