Lv Yunfu, Li Yejuan, Han XiaoYu, Liu Ning, Zeng Min
Department of General Surgery, Hainan General Hospital (Hainan Medical College Affiliated People's Hospital), Haikou, China.
Reproductive Medicine Center of Hainan Women and Children's Medical Center, Haikou, China.
Arch Med Sci. 2023 Aug 25;19(5):1569-1575. doi: 10.5114/aoms/170910. eCollection 2023.
To investigate the expression and treatment of chemokine CXCL12 and its receptor CXCR4/CXCR7.
The liver cirrhosis hypersplenism model of rats was made with CCL4, and then was detected by immunohistochemistry, Western blot and qRT-PCR.
The area of spleen fibrosis in the model group was significantly larger than that in the control group ( < 0.01), and the expression of CXCL12, CXCR4 and CXCR7 in the model group was significantly higher than that in the control group ( < 0.01).
CXCL12-CXCR4/CXCR7 is abnormally high in splenic fibrosis, and blocking its high expression can slow down the occurrence of hypersplenism.
探讨趋化因子CXCL12及其受体CXCR4/CXCR7的表达及治疗情况。
采用四氯化碳制备大鼠肝硬化脾功能亢进模型,然后通过免疫组织化学、蛋白质印迹法及实时荧光定量聚合酶链反应进行检测。
模型组脾纤维化面积显著大于对照组(<0.01),模型组CXCL12、CXCR4及CXCR7的表达显著高于对照组(<0.01)。
CXCL12-CXCR4/CXCR7在脾纤维化中异常高表达,阻断其高表达可减缓脾功能亢进的发生。