van Gelder P A, Klopper P J
Plast Reconstr Surg. 1979 Jul;64(1):59-64. doi: 10.1097/00006534-197907000-00011.
We made 44 microsurgical anastomoses in the femoral artery in rats. The healing was studied from one day to 4 months, by making an acrylic cast of the lumen of the femoral artery and the surrounding vessels, and examining the cast by scanning electron microscopy. The method proved to be easy and relatively quick, and it limited the possibility of artifacts. The high depth of focus permitted simultaneous study of the main lumen and of the perivascular structures after microvascular anastomoses.
我们在大鼠股动脉上进行了44次显微外科吻合术。通过制作股动脉管腔及周围血管的丙烯酸铸型,并利用扫描电子显微镜对铸型进行检查,从术后1天至4个月对愈合情况进行了研究。该方法简便且相对快速,还减少了产生伪像的可能性。高景深使得在微血管吻合术后能够同时研究主腔和血管周围结构。