超声引导下的淋巴结细针抽吸术用于评估人类接种疫苗后的生发中心反应。

Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans.

机构信息

Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.

The US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

出版信息

STAR Protoc. 2023 Dec 15;4(4):102576. doi: 10.1016/j.xpro.2023.102576. Epub 2023 Sep 20.

DOI:10.1016/j.xpro.2023.102576

PMID:37733596

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10519838/

Abstract

The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations to evaluate immunogens targeting germline cells. We describe steps for media and tube preparation and sample collection using an ultrasound-guided LN fine-needle aspiration procedure. This protocol is safe, quick, low-cost, and less invasive than excisional biopsy. For complete details on the use and execution of this protocol, please refer to Leggat et al. (2022)..

摘要

淋巴结 (LN) 是疫苗接种后免疫成熟的关键生物部位，因为它包含了几个对启动抗体反应至关重要的细胞群体。在这里，我们提供了一个从淋巴结中取样和分离细胞群体的方案，以评估针对生殖细胞的免疫原。我们描述了使用超声引导的 LN 细针抽吸程序进行培养基和管准备以及样本收集的步骤。与切除活检相比，该方案安全、快速、低成本且侵入性更小。有关此方案的使用和执行的完整详细信息，请参阅 Leggat 等人。(2022)。