Sun Xudong, Shan Yongjie, Jian Minghong, Wang Zhenxin
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, P. R. China.
Anal Chem. 2023 Oct 17;95(41):15146-15152. doi: 10.1021/acs.analchem.3c02973. Epub 2023 Sep 21.
The landscape of diagnostic assessments has experienced a paradigm shift driven by the advent of isothermal amplification techniques on point-of-care testing (POCT). The development of compact, portable isothermal amplification devices further emphasizes their transformative influence on diagnostic approaches. However, in prioritizing portability, these devices may exhibit limitations in functionality, rendering them less effective in addressing urgent public health emergencies during sudden pathogen outbreaks. In this paper, an efficient isothermal fluorescence amplification device has been fabricated for the rapid detection of pathogens during public health crises. The device features multichannel capability for simultaneous detection of various targets, integrates with the Internet of Medical Things (IoMT) for remote control and data uploading, and includes a deep learning-based batch processing system for rapid (9.4 ms) and accurate discrimination of pathogen type with excellent accuracy. The device has been successfully employed to simultaneously detect (SA) and methicillin-resistant (MRSA) with limits of detection (LODs) of 18 CFU/mL (SA) and 20 CFU/mL (MRSA) within 35 min by multiplex RPA assay and CRISPR/Cas12a-mediated nucleic acid detection assay.
随着即时检测（POCT）中恒温扩增技术的出现，诊断评估领域经历了范式转变。紧凑、便携式恒温扩增设备的发展进一步凸显了它们对诊断方法的变革性影响。然而，在优先考虑便携性的同时，这些设备可能在功能上存在局限性，在突发病原体爆发期间应对紧急公共卫生事件时效果欠佳。本文制造了一种高效的恒温荧光扩增设备，用于在公共卫生危机期间快速检测病原体。该设备具有多通道能力，可同时检测各种目标，与医疗物联网（IoMT）集成以进行远程控制和数据上传，并包括一个基于深度学习的批处理系统，能够以9.4毫秒的速度快速、准确地鉴别病原体类型，准确率极高。该设备已成功用于通过多重RPA分析和CRISPR/Cas12a介导的核酸检测分析，在35分钟内同时检测金黄色葡萄球菌（SA）和耐甲氧西林金黄色葡萄球菌（MRSA），检测限分别为18 CFU/mL（SA）和20 CFU/mL（MRSA）。
