Plant Cell Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, PO Box 1210, 1000, BE, Amsterdam, the Netherlands.
Plant Cell Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, PO Box 1210, 1000, BE, Amsterdam, the Netherlands.
Phytochemistry. 2023 Dec;216:113862. doi: 10.1016/j.phytochem.2023.113862. Epub 2023 Sep 19.
Several drought and salt tolerant phenotypes have been reported when overexpressing (OE) phospholipase C (PLC) genes across plant species. In contrast, a negative role for Arabidopsis PLC4 in salinity stress was recently proposed, showing that roots of PLC4-OE seedlings were more sensitive to NaCl while plc4 knock-out (KO) mutants were more tolerant. To investigate this apparent contradiction, and to analyse the phospholipid signalling responses associated with salinity stress, we performed root growth- and phospholipid analyses on plc4-KO and PLC4-OE seedlings subjected to salinity (NaCl) or osmotic (sorbitol) stress and compared these with wild type (WT). Only very minor differences between PLC4 mutants and WT were observed, which even disappeared after normalization of the data, while in soil, PLC4-OE plants were clearly more drought tolerant than WT plants, as was found earlier when overexpressing Arabidopsis PLC2, -3, -5, -7 or -9. We conclude that PLC4 plays no opposite role in salt-or osmotic stress and rather behaves like the other Arabidopsis PLCs.
当在跨物种中过表达（OE）磷脂酶 C（PLC）基因时，已经报道了几种耐旱和耐盐表型。相比之下，最近提出了拟南芥 PLC4 在盐胁迫中的负作用，表明 PLC4-OE 幼苗的根对 NaCl 更敏感，而 plc4 敲除（KO）突变体更耐受。为了研究这种明显的矛盾，并分析与盐胁迫相关的磷脂信号响应，我们对 PLC4-KO 和 PLC4-OE 幼苗进行了根生长和磷脂分析，这些幼苗受到盐（NaCl）或渗透（山梨醇）胁迫，并将其与野生型（WT）进行了比较。在 PLC4 突变体和 WT 之间仅观察到非常小的差异，甚至在数据归一化后这些差异也消失了，而在土壤中，PLC4-OE 植物比 WT 植物明显更耐旱，这与早期过表达拟南芥 PLC2、-3、-5、-7 或 -9 时的发现一致。我们得出结论，PLC4 没有在盐或渗透胁迫中起相反的作用，而是表现得像其他拟南芥 PLC 一样。
