College of Life Science and Technology, Central South University of Forestry and Technology, No. 498, South Shaoshan Road, Changsha 410004, Hunan Province, China.
College of Environmental Science and Engineering, Central South University of Forestry and Technology, No. 498, South Shaoshan Road, Changsha 410004, Hunan Province, China.
Sci Total Environ. 2024 Jan 1;906:167219. doi: 10.1016/j.scitotenv.2023.167219. Epub 2023 Sep 20.
Cadmium (Cd) pollution in soil is an important factor endangering plant growth and harming human health through food chains. Koelreuteria paniculata is an important woody plant for ecological restoration of Cd-contaminated soils. In this study, KpMIPS gene of K. paniculata was cloned, and the expressed protein (60 kDa) had 1-phosphate synthase activity. The results showed that KpMIPS significantly promoted root development of K. paniculata and Arabidopsis thaliana, reduced damage to the roots of Arabidopsis thaliana caused by Cd, and decreased transfer of Cd to the aboveground parts of K. paniculata and Arabidopsis thaliana . In the K. paniculata plants overexpressing KpMIPS integrity of the root cells was maintained and the content of pectin and phytic acid was significantly increased. Overexpression of KpMIPS increased the Cd accumulation in the roots and decreased the Cd content in the stems and leaves. Clearly, KpMIPS could regulate the contents of pectin and phytic acid in K. paniculata, thereby passivating Cd and enriching it in the root cell wall, reducing the transfer of free Cd to other parts of K. paniculata, and providing a positive regulatory effect on the Cd resistance of K. paniculata. The results of the study provide a technical introduction for the selection and genetic improvement of target genes regulating heavy metal resistance of plants in phytoremediation technology.
土壤中的镉（Cd）污染是通过食物链危害植物生长和人类健康的重要因素。栾树是受 Cd 污染土壤生态修复的重要木本植物。本研究克隆了栾树 KpMIPS 基因，其表达的蛋白（60 kDa）具有 1-磷酸合酶活性。结果表明，KpMIPS 显著促进了栾树和拟南芥的根系发育，降低了 Cd 对拟南芥根系的损伤，减少了 Cd 向地上部分的转移。在 KpMIPS 完整性表达的栾树植株中，维持了根细胞的完整性，显著增加了果胶和植酸的含量。KpMIPS 的过表达增加了根中的 Cd 积累，降低了茎和叶中的 Cd 含量。显然，KpMIPS 可以调节栾树中的果胶和植酸含量，从而使 Cd 失活并将其富集在根细胞壁中，减少游离 Cd 向栾树其他部位的转移，对栾树的 Cd 抗性提供正向调节作用。该研究结果为植物修复技术中重金属抗性目标基因的选择和遗传改良提供了技术介绍。
