College of Biochemical Engineering, Beijing Union University, Beijing, 100023, P.R. China.
Department of Physics and Astronomy, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
J Microbiol Biotechnol. 2023 Dec 28;33(12):1657-1670. doi: 10.4014/jmb.2304.04039. Epub 2023 Aug 21.
This study aimed to evaluate the effects of and isolated from human feces coordinating with inulin on the composition of gut microbiota and metabolic profiles in db/db mice. These supplements were administered to db/db mice for 12 weeks. The results showed that the coordinating with inulin group (LI) exhibited lower fasting blood glucose levels than the model control group (MC). Additionally, LI was found to enhance colon tissue and increase the levels of short-chain fatty acids. 16S rRNA sequencing revealed that the abundance of and , which were significantly increased in the MC group compared with NC group, were significantly decreased by the treatment of LI that also restored the key genera of the _NK4A136_group, , , , and . Untargeted metabolomics analysis showed that lotaustralin, 5-hydroxyindoleacetic acid, and 13(S)-HpODE were increased while L-phenylalanine and L-tryptophan were decreased in the MC group compared with the NC group. However, the intervention of LI reversed the levels of these metabolites in the intestine. Correlation analysis revealed that and _group were negatively correlated with 5-hydroxyindoleacetic acid and 13(S)-HpODE, but positively correlated with L-tryptophan. 13(S)-HpODE was involved in the "linoleic acid metabolism". L-tryptophan and 5-hydroxyindoleacetic acid were involved in "tryptophan metabolism" and "serotonergic synapse". These findings suggest that LI may alleviate type 2 diabetes symptoms by modulating the abundance of and to regulate the pathways of "linoleic acid metabolism", "serotonergic synapse", and" tryptophan metabolism". Our results provide new insights into prevention and treatment of type 2 diabetes.
本研究旨在评估来源于人粪便的 与菊粉协同作用对 db/db 小鼠肠道微生物组成和代谢谱的影响。这些补充剂被给予 db/db 小鼠 12 周。结果表明，与模型对照组（MC）相比，协同作用组（LI）空腹血糖水平较低。此外，LI 被发现增强结肠组织并增加短链脂肪酸水平。16S rRNA 测序显示，与 NC 组相比，MC 组中显著增加的 和 丰度，通过 LI 处理显著降低，LI 还恢复了 NK4A136 组的关键属， ， ， ，和 。非靶向代谢组学分析表明，与 NC 组相比，MC 组中洛塔司汀、5-羟色氨酸乙酸和 13(S)-HpODE 增加，而 L-苯丙氨酸和 L-色氨酸减少。然而，LI 的干预逆转了这些代谢物在肠道中的水平。相关性分析表明， 和 _group 与 5-羟色氨酸乙酸和 13(S)-HpODE 呈负相关，与 L-色氨酸呈正相关。13(S)-HpODE 参与“亚油酸代谢”。L-色氨酸和 5-羟色氨酸乙酸参与“色氨酸代谢”和“血清素能突触”。这些发现表明，LI 可能通过调节 和 的丰度来缓解 2 型糖尿病症状，从而调节“亚油酸代谢”、“血清素能突触”和“色氨酸代谢”途径。我们的研究结果为 2 型糖尿病的预防和治疗提供了新的思路。
