Host-Microbe Interactomics Group, Wageningen University & Research, Wageningen, The Netherlands.
Laboratory of Food Chemistry, Wageningen University & Research, Wageningen, The Netherlands.
Appl Environ Microbiol. 2021 Apr 13;87(9). doi: 10.1128/AEM.00122-21.
Synbiotics are food supplements that combine probiotics and prebiotics to synergistically elicit health benefits in the consumer. strains display high survival during transit through the mammalian gastrointestinal tract and were shown to have health-promoting properties. Growth on the fructose polysaccharide inulin is relatively uncommon in , and in this study we describe FosE, a plasmid-encoded β-fructosidase of strain Lp900 which has inulin-hydrolyzing properties. FosE contains an LPxTG-like motif involved in sortase-dependent cell wall anchoring but is also (partially) released in the culture supernatant. In addition, we examined the effect of diet supplementation with inulin on the intestinal persistence of Lp900 in adult male Wistar rats in diets with distinct calcium levels. Inulin supplementation in high-dietary-calcium diets significantly increased the intestinal persistence of Lp900, whereas this effect was not observed upon inulin supplementation of the low-calcium diet. Moreover, intestinal persistence of Lp900 was determined when provided as a probiotic (by itself) or as a synbiotic (i.e., in an inulin suspension) in rats that were fed unsupplemented diets containing the different calcium levels, revealing that the synbiotic administration increased bacterial survival and led to higher abundance of Lp900 in rats, particularly in a low-calcium-diet context. Our findings demonstrate that inulin supplementation can significantly enhance the intestinal delivery of Lp900 but that this effect strongly depends on calcium levels in the diet. Synbiotics combine probiotics with prebiotics to synergistically elicit a health benefit in the consumer. Previous studies have shown that prebiotics can selectively stimulate the growth in the intestine of specific bacterial strains. In synbiotic supplementations the prebiotics constituent could increase the intestinal persistence and survival of accompanying probiotic strain(s) and/or modulate the endogenous host microbiota to contribute to the synergistic enhancement of the health-promoting effects of the synbiotic constituents. Our study establishes a profound effect of dietary-calcium-dependent inulin supplementation on the intestinal persistence of inulin-utilizing Lp900 in rats. We also show that in rats on a low-dietary-calcium regime, the survival and intestinal abundance of Lp900 are significantly increased by administering it as an inulin-containing synbiotic. This study demonstrates that prebiotics can enhance the intestinal delivery of specific probiotics and that the prebiotic effect is profoundly influenced by the calcium content of the diet.
合生素是将益生菌和益生元结合起来，协同发挥对消费者健康益处的食品补充剂。研究表明， 株在通过哺乳动物胃肠道的转运过程中具有高存活率，并具有促进健康的特性。 在果糖多聚糖菊粉上的生长相对较少见，在本研究中，我们描述了 Lp900 菌株中质粒编码的 β-果糖苷酶 FosE，它具有菊粉水解特性。FosE 包含一个 LPxTG 样基序，该基序参与依赖于裂合酶的细胞壁锚定，但也（部分）释放到培养上清液中。此外，我们研究了在高膳食钙水平的饮食中补充菊粉对成年雄性 Wistar 大鼠肠道中 Lp900 持久性的影响。在高膳食钙饮食中补充菊粉显著增加了 Lp900 的肠道持久性，而在低膳食钙饮食中补充菊粉则没有观察到这种效果。此外，当在不添加钙的饮食中提供益生菌（单独）或共生菌（即在菊粉悬浮液中）时，我们确定了 Lp900 的肠道持久性，结果表明共生菌给药增加了细菌的存活率，并导致大鼠中 Lp900 的丰度更高，尤其是在低钙饮食环境中。我们的研究结果表明，菊粉补充剂可以显著增强 Lp900 的肠道传递，但这种效果强烈依赖于饮食中的钙水平。合生素将益生菌与益生元结合起来，协同发挥对消费者的健康益处。先前的研究表明，益生元可以选择性地刺激特定细菌菌株在肠道中的生长。在共生素补充中，益生元成分可以增加伴随益生菌菌株的肠道持久性和存活率，和/或调节内源性宿主微生物群，有助于协同增强共生素成分的健康促进作用。我们的研究建立了饮食中钙依赖性菊粉补充对大鼠肠道中利用菊粉的 Lp900 持久性的深远影响。我们还表明，在低膳食钙水平的大鼠中，通过给予含有菊粉的共生体，Lp900 的存活率和肠道丰度显著增加。这项研究表明，益生元可以增强特定益生菌的肠道传递，并且这种益生元的效果受到饮食中钙含量的极大影响。
