Finck S, Walker J, Vaccaro P S, Kakos G S, Howanitz E P, Cornhill J F
J Surg Res. 1986 Oct;41(4):419-24. doi: 10.1016/0022-4804(86)90056-9.
The effect of ischemia on arterial endothelial permeability was assessed by surgically interrupting arterial blood flow for 45 min in the left carotid artery of 12 foxhounds. The right carotid artery served as control. Twenty-four hours before sacrificing the animals at 1 day, 1 week, 1 month, and 3 months postoperatively. Evans blue dye (1.5 ml/kg) was administered intravenously. Carotid arteries were harvested, opened, and scanned with a reflectometer to measure Evans blue dye uptake, and scanning electron microscopy was performed on a section of tissue from each harvested vessel. A statistically significant increase in permeability of the ischemic vessel occurred at 1 day (79 +/- 42% (SD], 1 week (186 +/- 75%), and 1 month (229 +/- 125%), but was not present at 3 months (7 +/- 8%) postinjury. Scanning electron microscopic examination of all specimens was essentially normal. This study demonstrates that arterial endothelium has increased permeability at 1 month following a brief ischemic period. What effect this ischemia-induced endothelial dysfunction will have on lipid uptake by the arterial wall will be the subject of future study.
通过手术阻断12只猎狐犬左颈动脉的动脉血流45分钟，评估缺血对动脉内皮通透性的影响。右颈动脉作为对照。在术后1天、1周、1个月和3个月处死动物前24小时，静脉注射伊文思蓝染料（1.5 ml/kg）。采集颈动脉，打开并用反射仪扫描以测量伊文思蓝染料摄取量，并对每个采集血管的一段组织进行扫描电子显微镜检查。缺血血管的通透性在损伤后1天（79±42%（标准差））、1周（186±75%）和1个月（229±125%）出现统计学上的显著增加，但在3个月时（7±8%）未出现。所有标本的扫描电子显微镜检查基本正常。本研究表明，短暂缺血期后1个月动脉内皮通透性增加。这种缺血诱导的内皮功能障碍对动脉壁脂质摄取的影响将是未来研究的主题。
