用伊文思蓝染料和碘125标记白蛋白评估动脉化静脉移植物的通透性。

Evaluation of arterialized vein graft permeability with Evans blue dye and iodine 125-labeled albumin.

作者信息

Finck S J, Mashburn J P, Kottke B A, Orszulak T A

机构信息

Section of Cardiovascular and Thoracic Surgery, Mayo Clinic Jacksonville, Florida 32224.

出版信息

Ann Thorac Surg. 1989 Nov;48(5):646-50. doi: 10.1016/0003-4975(89)90781-9.

DOI:10.1016/0003-4975(89)90781-9

PMID:2818053

Abstract

This study measured endothelial permeability, quantitatively (with iodine 125-labeled albumin) and qualitatively (with Evans blue dye) in arterialized vein grafts in 9 adult dogs. In each dog, the right common carotid artery was ligated and arterial flow was reconstituted with a 5-cm bypass graft of right external jugular vein. Twenty-four hours before the dogs were killed, each dog received both 125I-labeled albumin (8 microCi/kg) and Evans blue dye (15 mg/kg) intravenously. After the dogs' death, each arterialized vein graft and each left external jugular vein (as control) was harvested and studied. Arterialized vein graft permeability was significantly increased over control at 1, 3, and 6 months (3 dogs at each interval). Scanning electron microscopy revealed confluent endothelium in all specimens. This increased permeability may play a role in vein graft atherosclerosis.

摘要

本研究对9只成年犬的动脉化静脉移植物的内皮通透性进行了定量（使用碘125标记的白蛋白）和定性（使用伊文思蓝染料）测量。在每只犬中，结扎右侧颈总动脉，并用一段5厘米长的右侧颈外静脉搭桥移植物重建动脉血流。在处死犬前24小时，每只犬静脉注射125I标记的白蛋白（8微居里/千克）和伊文思蓝染料（15毫克/千克）。犬死亡后，采集每只动脉化静脉移植物和每只左侧颈外静脉（作为对照）并进行研究。在1个月、3个月和6个月时（每个时间间隔3只犬），动脉化静脉移植物的通透性比对照显著增加。扫描电子显微镜显示所有标本中的内皮细胞融合。这种通透性增加可能在静脉移植物动脉粥样硬化中起作用。